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Literature summary for 1.7.1.16 extracted from
- A novel cold-adapted nitroreductase from Psychrobacter sp. ANT206 Heterologous expression, characterization and nitrobenzene reduction capacity (2019), Enzyme Microb. Technol., 131, 109434 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 146.27% activity at 10 mM | Psychrobacter sp. ANT206 |  |
| GSH | 118.3% activity at 10 mM | Psychrobacter sp. ANT206 | |
| NaCl | the enzyme exhibits the highest activity (136.4%) at 1.0M NaCl | Psychrobacter sp. ANT206 | |
| PEG 20000 | 113.3% activity at 5 mM | Psychrobacter sp. ANT206 | |
| Tween 20 | 120.5% activity at 0.2% (v/v) | Psychrobacter sp. ANT206 | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Psychrobacter sp. ANT206 |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme maintains more than 60% of its initial activity after incubation at 3.5 M NaCl | Psychrobacter sp. ANT206 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 73.3% residual activity at 0.2% (v/v) | Psychrobacter sp. ANT206 | |
| Cu2+ | 35% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
| EDTA | 56.7% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
| Fe2+ | 78.9% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
| Fe3+ | 40% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
| Ni2+ | 46.7% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
| SDS | 16.7% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
| Thiourea | 35.7% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
| Triton X-100 | 39.6% residual activity at 0.2% (v/v) | Psychrobacter sp. ANT206 | |
| Urea | 42.8% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
| Zn2+ | 49.8% residual activity at 10 mM | Psychrobacter sp. ANT206 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00182 | - |
nitrobenzene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 0.00202 | - |
4-nitrophenol | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 0.0043 | - |
4-nitrochlorobenzene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 0.00513 | - |
2-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 0.00567 | - |
2-chloro-6-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 0.00592 | - |
3-bromo-4-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 161.7% activity at 10 mM | Psychrobacter sp. ANT206 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrobenzene + 3 NADPH + 3 H+ | Psychrobacter sp. ANT206 | - |
aniline + 3 NADP+ + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Psychrobacter sp. ANT206 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Psychrobacter sp. ANT206 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51.59 | - |
purified enzyme, at pH 7.0 and 25°C | Psychrobacter sp. ANT206 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloro-6-nitrotoluene + 3 NADPH + 3 H+ | - |
Psychrobacter sp. ANT206 | 3-chloro-2-methylaniline + 3 NADP+ + 2 H2O | - |
? | |
| 2-nitrotoluene + 3 NADPH + 3 H+ | - |
Psychrobacter sp. ANT206 | 2-toluidine + 3 NADP+ + 2 H2O | - |
? | |
| 3-bromo-4-nitrotoluene + 3 NADPH + 3 H+ | - |
Psychrobacter sp. ANT206 | 2-bromo-4-methylaniline + 3 NADP+ + 2 H2O | - |
? | |
| 4-nitrochlorobenzene + 3 NADPH + 3 H+ | - |
Psychrobacter sp. ANT206 | 4-chloroaniline + 3 NADP+ + 2 H2O | - |
? | |
| 4-nitrophenol + 3 NADPH + 3 H+ | - |
Psychrobacter sp. ANT206 | 4-aminophenol + 3 NADP+ + 2 H2O | - |
? | |
| nitrobenzene + 3 NADH + 3 H+ | 30% activity compared to 3 NADPH | Psychrobacter sp. ANT206 | aniline + 3 NAD+ + 2 H2O | - |
? | |
| nitrobenzene + 3 NADPH + 3 H+ | - |
Psychrobacter sp. ANT206 | aniline + 3 NADP+ + 2 H2O | - |
? | |
| nitrobenzene + 3 NADPH + 3 H+ | 100% activity with 3 NADPH | Psychrobacter sp. ANT206 | aniline + 3 NADP+ + 2 H2O | - |
? | |
| nitrobenzene + FMN | 80% activity compared to 3 NADPH | Psychrobacter sp. ANT206 | aniline + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28830, calculated from amino acid sequence | Psychrobacter sp. ANT206 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NTR | - |
Psychrobacter sp. ANT206 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Psychrobacter sp. ANT206 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
40 | the enzyme maintains 46.9% of the optimum activity at 0°C and show more than 50% activity between 10 and 45°C | Psychrobacter sp. ANT206 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | the enzyme is very stable at 30°C, and the enzyme activity sharply reduces as the temperature increases. The half-life of the enzyme is 40 min at 50°C, approximately | Psychrobacter sp. ANT206 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 64.25 | - |
3-bromo-4-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 66.82 | - |
2-chloro-6-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 70.45 | - |
2-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 87.14 | - |
4-nitrochlorobenzene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 223.59 | - |
4-nitrophenol | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 256.54 | - |
nitrobenzene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Psychrobacter sp. ANT206 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | more than 56% activity between pH 4.0 and 8.0 | Psychrobacter sp. ANT206 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | the enzyme retains good stability at pH 4.0-9.0 after the incubation at 25°C for 30 min | Psychrobacter sp. ANT206 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Psychrobacter sp. ANT206 | |
| NADH | - |
Psychrobacter sp. ANT206 | |
| NADPH | best cofactor | Psychrobacter sp. ANT206 | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Psychrobacter sp. ANT206 | calculated from amino acid sequence | - |
6.9 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10850 | - |
3-bromo-4-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 11780 | - |
2-chloro-6-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 13730 | - |
2-nitrotoluene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 20270 | - |
4-nitrochlorobenzene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 110400 | - |
4-nitrophenol | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
| 140960 | - |
nitrobenzene | at pH 7.0 and 25°C | Psychrobacter sp. ANT206 | |
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