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Literature summary for 1.7.1.17 extracted from

  • Mercier, C.; Chalansonnet, V.; Orenga, S.; Gilbert, C.
    Characteristics of major Escherichia coli reductases involved in aerobic nitro and azo reduction (2013), J.Appl. Microbiol., 115, 1012-1022 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene azoR, recombinant overexpression of His-tagged enzyme in Escherichia coli strain XL1-Blue Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information deletion of the azoR gene in Escherichia coli strain MG1655 has no strong influence on the 7NCCA reduction of the cells even though the pure enzyme displays a clear nitroreductase activity with this compound Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ Escherichia coli i.e. azo dye methyl red anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+
-
?
7-nitrocoumarin-3-carboxylic acid + NADH + H+ Escherichia coli
-
7-aminocoumarin-3-carboxylic acid + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P41407
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ ping-pong reaction mechanism. Enzymatic reaction involves an electron transfer from nicotinamide dinucleotide (NADH) to flavin mononucleotide (FMN) (ping) followed by a transfer from FMNH2 to the substrate (pong), key role of flavin Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ i.e. azo dye methyl red Escherichia coli anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+
-
?
2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ i.e. azo dye methyl red, via a diazene intermediate Escherichia coli anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+
-
?
7-nitrocoumarin-3-carboxylic acid + 2 NADH + 2 H+ via 7-nitrosocoumarin-3-carboxylic acid and 7-hydroxylaminocoumarin-3-carboxylic acid intermediates Escherichia coli 7-aminocoumarin-3-carboxylic acid + NAD+ + H2O
-
?
7-nitrocoumarin-3-carboxylic acid + NADH + H+
-
Escherichia coli 7-aminocoumarin-3-carboxylic acid + NAD+ + H2O
-
?

Synonyms

Synonyms Comment Organism
AzoR
-
Escherichia coli
azoreductase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN enzyme AzoR is strictly FMN dependent Escherichia coli
NADH
-
Escherichia coli