Cloned (Comment) | Organism |
---|---|
gene azoR, recombinant overexpression of His-tagged enzyme in Escherichia coli strain XL1-Blue | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the azoR gene in Escherichia coli strain MG1655 has no strong influence on the 7NCCA reduction of the cells even though the pure enzyme displays a clear nitroreductase activity with this compound | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ | Escherichia coli | i.e. azo dye methyl red | anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ | - |
? | |
7-nitrocoumarin-3-carboxylic acid + NADH + H+ | Escherichia coli | - |
7-aminocoumarin-3-carboxylic acid + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P41407 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ | ping-pong reaction mechanism. Enzymatic reaction involves an electron transfer from nicotinamide dinucleotide (NADH) to flavin mononucleotide (FMN) (ping) followed by a transfer from FMNH2 to the substrate (pong), key role of flavin | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ | i.e. azo dye methyl red | Escherichia coli | anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ | - |
? | |
2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ | i.e. azo dye methyl red, via a diazene intermediate | Escherichia coli | anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ | - |
? | |
7-nitrocoumarin-3-carboxylic acid + 2 NADH + 2 H+ | via 7-nitrosocoumarin-3-carboxylic acid and 7-hydroxylaminocoumarin-3-carboxylic acid intermediates | Escherichia coli | 7-aminocoumarin-3-carboxylic acid + NAD+ + H2O | - |
? | |
7-nitrocoumarin-3-carboxylic acid + NADH + H+ | - |
Escherichia coli | 7-aminocoumarin-3-carboxylic acid + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AzoR | - |
Escherichia coli |
azoreductase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | enzyme AzoR is strictly FMN dependent | Escherichia coli | |
NADH | - |
Escherichia coli |