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Literature summary for 1.7.1.17 extracted from

  • Ooi, T.; Shibata, T.; Sato, R.; Ohno, H.; Kinoshita, S.; Thuoc, T.; Taguchi, S.
    An azoreductase, aerobic NADH-dependent flavoprotein discovered from Bacillus sp. Functional expression and enzymatic characterization (2007), Appl. Microbiol. Biotechnol., 75, 377-386 .
    View publication on PubMed
Search for more literature for 1.7.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. B29

General Stability

General Stability Organism
activity is oxygen insensitive Bacillus sp. B29

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 gel filtration Bacillus sp. B29

Organism

Organism UniProt Comment Textmining
Bacillus sp. B29 Q0WXX2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.3
-
 substrate acid red 88, pH 7.4, 30°C Bacillus sp. B29
11.4
-
 substrate orange I, pH 7.4, 30°C Bacillus sp. B29
24.1
-
 substrate 1-(2-pyridylazo)-2-naphthol,pH 7.4, 30°C Bacillus sp. B29
26.6
-
 substrate 4'-(dimethylamino)-azobenzene-2-carboxylic acid, pH 7.4, 30°C Bacillus sp. B29

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2-pyridylazo)-2-naphthol + 2 NADH + 2 H+
-
 Bacillus sp. B29 pyridin-2-amine + 1-aminonaphthalen-2-ol + 2 NAD+
-
 ?
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Bacillus sp. B29 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?
acid red 88 + 2 NADH + 2 H+
-
 Bacillus sp. B29 4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NAD+
-
 ?
orange I + 2 NADH + 2 H+
-
 Bacillus sp. B29 4-aminobenzene-1-sulfonic acid + 4-aminonaphthalen-1-ol + 2 NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 22766, calculated, 2 * 23000, SDS-PAGE Bacillus sp. B29

Synonyms

Synonyms Comment Organism
AzrA
-
 Bacillus sp. B29

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65 80 broad Bacillus sp. B29

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable for at least 1 h Bacillus sp. B29
65
-
 1 h, more than 50% residual activity Bacillus sp. B29

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 30°C, 1 h, more than 90% of maximum activity Bacillus sp. B29

Cofactor

Cofactor Comment Organism Structure
FMN contains 2 molecules of oxidized FMN per dimer Bacillus sp. B29
NADH
-
 Bacillus sp. B29