Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
N121Q | 115% of wild-type activity | Enterococcus faecalis |
R21G/N121A | 136% of wild-type activity | Enterococcus faecalis |
R21G/N121Q | 171% of wild-type activity | Enterococcus faecalis |
R21K | 80% of wild-type activity | Enterococcus faecalis |
R21K/N121A | 41% of wild-type activity | Enterococcus faecalis |
R21K/N121Q | 40% of wild-type activity | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant R21G/N121A, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.016 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant R21G/N121Q, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.02 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant R21K, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.024 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | wild-type, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.029 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant N121Q, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.031 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant R21K/N121A, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.034 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant R21K/N121Q, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.08 | - |
NADH | mutant R21G/N121A, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.115 | - |
NADH | mutant R21K/N121Q, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.122 | - |
NADH | mutant R21K, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.143 | - |
NADH | wild-type, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.19 | - |
NADH | mutant R21G/N121Q, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.267 | - |
NADH | mutant R21K/N121Q, pH 7.1, temperature not specified in the publication | Enterococcus faecalis | |
0.371 | - |
NADH | mutant N121Q, pH 7.1, temperature not specified in the publication | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q831B2 | - |
- |
Enterococcus faecalis ATCC 700802 | Q831B2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ | i.e. methyl red | Enterococcus faecalis | 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ | - |
? | |
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ | i.e. methyl red | Enterococcus faecalis ATCC 700802 | 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
AzoR | - |
Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Enterococcus faecalis |