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Literature summary for 1.7.1.17 extracted from

  • Sun, J.; Kweon, O.; Jin, J.; He, G.; Li, X.; Cerniglia, C.; Chen, H.
    Mutation network-based understanding of pleiotropic and epistatic mutational behavior of Enterococcus faecalis FMN-dependent azoreductase (2017), Biochem. Biophys. Rep., 12, 240-244 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecalis

Protein Variants

Protein Variants Comment Organism
N121Q 115% of wild-type activity Enterococcus faecalis
R21G/N121A 136% of wild-type activity Enterococcus faecalis
R21G/N121Q 171% of wild-type activity Enterococcus faecalis
R21K 80% of wild-type activity Enterococcus faecalis
R21K/N121A 41% of wild-type activity Enterococcus faecalis
R21K/N121Q 40% of wild-type activity Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21G/N121A, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.016
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21G/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.02
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21K, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.024
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid wild-type, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.029
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.031
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21K/N121A, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.034
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21K/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.08
-
NADH mutant R21G/N121A, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.115
-
NADH mutant R21K/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.122
-
NADH mutant R21K, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.143
-
NADH wild-type, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.19
-
NADH mutant R21G/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.267
-
NADH mutant R21K/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
0.371
-
NADH mutant N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q831B2
-
-
Enterococcus faecalis ATCC 700802 Q831B2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
?
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 700802 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Enterococcus faecalis

Synonyms

Synonyms Comment Organism
AzoR
-
Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Enterococcus faecalis