Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | cofactor roseoflavin mononucleotide, pH 7.4, 30°C | Escherichia coli | |
0.027 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | cofactor FMN, pH 7.4, 30°C | Escherichia coli | |
0.26 | - |
NADH | cofactor FMN, pH 7.4, 30°C | Escherichia coli | |
0.269 | - |
NADH | cofactor roseoflavin mononucleotide, pH 7.4, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P41407 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ | i.e. methyl red | Escherichia coli | 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AzoR | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli | |
additional information | no cofactor: 8-amino-8-demethyl-riboflavin mononucleotide | Escherichia coli | |
roseoflavin mononucleotide | roseoflavin mononucleotide binds to the AzoR apoenzyme with an even higher affinity compared to that of the natural cofactor FMN. Roseoflavin mononucleotide binding does not affect the overall topology of the enzyme and also does not interfere with dimerization of AzoR. With roseoflavin mononucleotide, the holoenzyme shiws about 30% of wild-type activity | Escherichia coli |