Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
W105A | complete loss of both affinity for FMN and enzyme activity | Enterococcus faecalis |
W105F | 31fold decrease in vmax value, Km value similar to wild-type | Enterococcus faecalis |
W105G | complete loss of both affinity for FMN and enzyme activity | Enterococcus faecalis |
W105H | 8fold decrease in vmax value, Km value similar to wild-type | Enterococcus faecalis |
W105Q | 68fold decrease in vmax value, Km value similar to wild-type | Enterococcus faecalis |
W105Y | 22% reduction in vmax value | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0247 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant W105F, pH 7.1, 23°C | Enterococcus faecalis | |
0.0251 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant W105H, pH 7.1, 23°C | Enterococcus faecalis | |
0.0254 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | wild-type, pH 7.1, 23°C | Enterococcus faecalis | |
0.0276 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant W105Y, pH 7.1, 23°C | Enterococcus faecalis | |
0.0282 | - |
4'-(dimethylamino)-azobenzene-2-carboxylic acid | mutant W105Q, pH 7.1, 23°C | Enterococcus faecalis | |
0.158 | - |
NADH | wild-type, pH 7.1, 23°C | Enterococcus faecalis | |
0.166 | - |
NADH | mutant W105Y, pH 7.1, 23°C | Enterococcus faecalis | |
0.169 | - |
NADH | mutant W105Q, pH 7.1, 23°C | Enterococcus faecalis | |
0.171 | - |
NADH | mutant W105H, pH 7.1, 23°C | Enterococcus faecalis | |
0.175 | - |
NADH | mutant W105F, pH 7.1, 23°C | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q831B2 | - |
- |
Enterococcus faecalis ATCC 700802 | Q831B2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ | i.e. methyl red | Enterococcus faecalis | 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ | - |
? | |
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ | i.e. methyl red | Enterococcus faecalis ATCC 700802 | 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AzoA | - |
Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | residues Trp105, Asn106, Leu107, Gly150, Gly151, Tyr153, Asn121 and Tyr129 are involved in binding the FMN isoalloxazine ring. Residue Trp105 is involved in FMN stabilization and destabilization | Enterococcus faecalis | |
NADH | - |
Enterococcus faecalis |