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Literature summary for 1.7.1.17 extracted from

  • Chen, H.; Xu, H.; Kweon, O.; Chen, S.; Cerniglia, C.
    Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysis (2008), Microbiology, 154, 2659-2667 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecalis

Protein Variants

Protein Variants Comment Organism
W105A complete loss of both affinity for FMN and enzyme activity Enterococcus faecalis
W105F 31fold decrease in vmax value, Km value similar to wild-type Enterococcus faecalis
W105G complete loss of both affinity for FMN and enzyme activity Enterococcus faecalis
W105H 8fold decrease in vmax value, Km value similar to wild-type Enterococcus faecalis
W105Q 68fold decrease in vmax value, Km value similar to wild-type Enterococcus faecalis
W105Y 22% reduction in vmax value Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0247
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant W105F, pH 7.1, 23°C Enterococcus faecalis
0.0251
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant W105H, pH 7.1, 23°C Enterococcus faecalis
0.0254
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid wild-type, pH 7.1, 23°C Enterococcus faecalis
0.0276
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant W105Y, pH 7.1, 23°C Enterococcus faecalis
0.0282
-
4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant W105Q, pH 7.1, 23°C Enterococcus faecalis
0.158
-
NADH wild-type, pH 7.1, 23°C Enterococcus faecalis
0.166
-
NADH mutant W105Y, pH 7.1, 23°C Enterococcus faecalis
0.169
-
NADH mutant W105Q, pH 7.1, 23°C Enterococcus faecalis
0.171
-
NADH mutant W105H, pH 7.1, 23°C Enterococcus faecalis
0.175
-
NADH mutant W105F, pH 7.1, 23°C Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q831B2
-
-
Enterococcus faecalis ATCC 700802 Q831B2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
?
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 700802 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
?

Synonyms

Synonyms Comment Organism
AzoA
-
Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
FMN residues Trp105, Asn106, Leu107, Gly150, Gly151, Tyr153, Asn121 and Tyr129 are involved in binding the FMN isoalloxazine ring. Residue Trp105 is involved in FMN stabilization and destabilization Enterococcus faecalis
NADH
-
Enterococcus faecalis