Crystallization (Comment) | Organism |
---|---|
in complex with FMN, sitting drop vapor diffusion method, using 0.17 M ammonium sulfate and 25.2% (w/v) PEG 4000 | Alkalihalobacillus wakoensis |
Protein Variants | Comment | Organism |
---|---|---|
W60D | the mutant retains more than 10% of wild type activity | Alkalihalobacillus wakoensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
menadione | mutant enzyme W60A, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
0.061 | - |
1,2-naphthoquinone | wild type enzyme, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
0.062 | - |
1,2-naphthoquinone | mutant enzyme W60A, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
0.064 | - |
1,4-Naphthoquinone | mutant enzyme W60A, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
0.069 | - |
menadione | wild type enzyme, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
0.097 | - |
1,4-Naphthoquinone | wild type enzyme, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
0.115 | - |
Orange I | wild type enzyme, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
0.157 | - |
Orange I | mutant enzyme W60A, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus wakoensis | A0A455X8R2 | - |
- |
Alkalihalobacillus wakoensis A01 | A0A455X8R2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Alkalihalobacillus wakoensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-naphthoquinone + 2 NADH + 2 H+ | - |
Alkalihalobacillus wakoensis | ? | - |
? | |
1,2-naphthoquinone + 2 NADH + 2 H+ | - |
Alkalihalobacillus wakoensis A01 | ? | - |
? | |
1,4-naphthoquinone + 2 NADH + 2 H+ | - |
Alkalihalobacillus wakoensis | ? | - |
? | |
1,4-naphthoquinone + 2 NADH + 2 H+ | - |
Alkalihalobacillus wakoensis A01 | ? | - |
? | |
menadione + 2 NADH + 2 H+ | - |
Alkalihalobacillus wakoensis | ? | - |
? | |
menadione + 2 NADH + 2 H+ | - |
Alkalihalobacillus wakoensis A01 | ? | - |
? | |
additional information | the enzyme shows a negligible ability to use molecular oxygen as an electron acceptor | Alkalihalobacillus wakoensis | ? | - |
- |
|
additional information | the enzyme shows a negligible ability to use molecular oxygen as an electron acceptor | Alkalihalobacillus wakoensis A01 | ? | - |
- |
|
Orange I + 2 NADH + 2 H+ | - |
Alkalihalobacillus wakoensis | ? | - |
? | |
Orange I + 2 NADH + 2 H+ | - |
Alkalihalobacillus wakoensis A01 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Alkalihalobacillus wakoensis |
Synonyms | Comment | Organism |
---|---|---|
AzoA | - |
Alkalihalobacillus wakoensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
71 | - |
apparent melting temperature | Alkalihalobacillus wakoensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
Orange I | wild type enzyme, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
1.06 | - |
Orange I | mutant enzyme W60A, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
67 | - |
1,2-naphthoquinone | mutant enzyme W60A, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
111 | - |
1,2-naphthoquinone | wild type enzyme, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
136 | - |
menadione | mutant enzyme W60A, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
152 | - |
1,4-Naphthoquinone | mutant enzyme W60A, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
340 | - |
1,4-Naphthoquinone | wild type enzyme, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis | |
387 | - |
menadione | wild type enzyme, at pH 8.5 and 25°C | Alkalihalobacillus wakoensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Alkalihalobacillus wakoensis | |
NADH | the enzyme is strictly NADH-dependent | Alkalihalobacillus wakoensis |