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Literature summary for 1.7.1.17 extracted from

  • Qi, J.; Anke, M.; Szymanska, K.; Tischler, D.
    Immobilization of Rhodococcus opacus 1CP azoreductase to obtain azo dye degrading biocatalysts operative at acidic pH (2017), Int. Biodeter. Biodegrad., 118, 89-94 .
No PubMed abstract available
Search for more literature for 1.7.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus opacus

General Stability

General Stability Organism
non-immobilized enzyme shows a constant decrease in activity to become entirely inactive after 35 days, unlike activities of the immobilized biocatalysts (meso-cellular foams and mesoporous silica) which decline significantly during the first week and then they stabilize to retain ca. 27%-42% of the initial enzyme activity after 63 days Rhodococcus opacus

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus
-
-
-
Rhodococcus opacus 1CP
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Allura Red + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus ?
-
 ?
Allura Red + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus 1CP ?
-
 ?
Brilliant Black + NADH + H+ low activity Rhodococcus opacus ?
-
 ?
Brilliant Black + NADH + H+ low activity Rhodococcus opacus 1CP ?
-
 ?
methyl orange + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus ?
-
 ?
methyl orange + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus 1CP ?
-
 ?
methyl red + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus ?
-
 ?
methyl red + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus 1CP ?
-
 ?
additional information free enzyme has no activity with azobenzene, 4-phenylazophenol, Allura Red, Amido Black, Reactive Orange, Para Red, Chlorazol Black and Methyl Orange Rhodococcus opacus ?
-
-
additional information free enzyme has no activity with azobenzene, 4-phenylazophenol, Allura Red, Amido Black, Reactive Orange, Para Red, Chlorazol Black and Methyl Orange Rhodococcus opacus 1CP ?
-
-
Reactive Orange + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Rhodococcus opacus

Synonyms

Synonyms Comment Organism
azoreductase
-
 Rhodococcus opacus
AzoRo
-
 Rhodococcus opacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhodococcus opacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Rhodococcus opacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 the mesoporous silicas-immobilized enzyme is active after incubation at pH 4.0 for 60 h Rhodococcus opacus

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Rhodococcus opacus