Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Shewanella oneidensis |
Protein Variants | Comment | Organism |
---|---|---|
F117A | the mutant shows 208% activity with Methyl Red and 176% activity with NADH compared to the wild type enzyme | Shewanella oneidensis |
F161A | inactive | Shewanella oneidensis |
G140F | inactive | Shewanella oneidensis |
G141F | inactive | Shewanella oneidensis |
H143A | the mutant shows 19% activity with Methyl Red and 76% activity with NADH compared to the wild type enzyme | Shewanella oneidensis |
N96A | the mutant shows 9% activity with Methyl Red and 37% activity with NADH compared to the wild type enzyme | Shewanella oneidensis |
R139A | the mutant shows 63% activity with Methyl Red and 78% activity with NADH compared to the wild type enzyme | Shewanella oneidensis |
R59A | the mutant shows 70% activity with Methyl Red and 83% activity with NADH compared to the wild type enzyme | Shewanella oneidensis |
S16A | the mutant shows 29% activity with Methyl Red and 49% activity with NADH compared to the wild type enzyme | Shewanella oneidensis |
Y119A | the mutant shows 59% activity with Methyl Red and 44% activity with NADH compared to the wild type enzyme | Shewanella oneidensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01155 | - |
Methyl red | wild type enzyme, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.01603 | - |
Methyl red | mutant enzyme F117A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.01928 | - |
Methyl red | mutant enzyme Y119A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.02714 | - |
NADH | mutant enzyme F117A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.0296 | - |
Methyl red | mutant enzyme R59A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.04338 | - |
NADH | mutant enzyme H143A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.04809 | - |
Methyl red | mutant enzyme R139A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.06872 | - |
Methyl red | mutant enzyme H143A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.07676 | - |
Methyl red | mutant enzyme S16A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.09315 | - |
Methyl red | mutant enzyme N96A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.18777 | - |
NADH | wild type enzyme, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.2241 | - |
NADH | mutant enzyme R139A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.3158 | - |
NADH | mutant enzyme N96A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.3433 | - |
NADH | mutant enzyme R59A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.5307 | - |
NADH | mutant enzyme S16A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.6551 | - |
NADH | mutant enzyme Y119A, at pH 7.4 and 30°C | Shewanella oneidensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella oneidensis | Q8E990 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Shewanella oneidensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Methyl Red + NADH + H+ | - |
Shewanella oneidensis | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AzoR | - |
Shewanella oneidensis |
azoreductase | - |
Shewanella oneidensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2900 | - |
Methyl red | mutant enzyme N96A, at pH 7.4 and 30°C | Shewanella oneidensis | |
6350 | - |
Methyl red | mutant enzyme H143A, at pH 7.4 and 30°C | Shewanella oneidensis | |
9800 | - |
Methyl red | mutant enzyme S16A, at pH 7.4 and 30°C | Shewanella oneidensis | |
14420 | - |
NADH | mutant enzyme N96A, at pH 7.4 and 30°C | Shewanella oneidensis | |
17170 | - |
NADH | mutant enzyme Y119A, at pH 7.4 and 30°C | Shewanella oneidensis | |
19180 | - |
NADH | mutant enzyme S16A, at pH 7.4 and 30°C | Shewanella oneidensis | |
20020 | - |
Methyl red | mutant enzyme Y119A, at pH 7.4 and 30°C | Shewanella oneidensis | |
21480 | - |
Methyl red | mutant enzyme R139A, at pH 7.4 and 30°C | Shewanella oneidensis | |
23680 | - |
Methyl red | mutant enzyme R59A, at pH 7.4 and 30°C | Shewanella oneidensis | |
29390 | - |
NADH | mutant enzyme H143A, at pH 7.4 and 30°C | Shewanella oneidensis | |
30200 | - |
NADH | mutant enzyme R139A, at pH 7.4 and 30°C | Shewanella oneidensis | |
32130 | - |
NADH | mutant enzyme R59A, at pH 7.4 and 30°C | Shewanella oneidensis | |
33860 | - |
Methyl red | wild type enzyme, at pH 7.4 and 30°C | Shewanella oneidensis | |
33900 | - |
NADH | wild type enzyme, at pH 7.4 and 30°C | Shewanella oneidensis | |
68230 | - |
NADH | mutant enzyme F117A, at pH 7.4 and 30°C | Shewanella oneidensis | |
70360 | - |
Methyl red | mutant enzyme F117A, at pH 7.4 and 30°C | Shewanella oneidensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Shewanella oneidensis | |
NADH | - |
Shewanella oneidensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
Methyl red | mutant enzyme N96A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.03 | - |
NADH | mutant enzyme Y119A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.04 | - |
NADH | mutant enzyme S16A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.05 | - |
NADH | mutant enzyme N96A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.07 | - |
NADH | mutant enzyme H143A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.09 | - |
Methyl red | mutant enzyme H143A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.09 | - |
NADH | mutant enzyme R59A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.13 | - |
Methyl red | mutant enzyme S16A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.14 | - |
NADH | mutant enzyme R139A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.18 | - |
NADH | wild type enzyme, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.45 | - |
Methyl red | mutant enzyme R139A, at pH 7.4 and 30°C | Shewanella oneidensis | |
0.8 | - |
Methyl red | mutant enzyme R59A, at pH 7.4 and 30°C | Shewanella oneidensis | |
1.04 | - |
Methyl red | mutant enzyme Y119A, at pH 7.4 and 30°C | Shewanella oneidensis | |
2.51 | - |
NADH | mutant enzyme F117A, at pH 7.4 and 30°C | Shewanella oneidensis | |
2.93 | - |
Methyl red | wild type enzyme, at pH 7.4 and 30°C | Shewanella oneidensis | |
4.39 | - |
Methyl red | mutant enzyme F117A, at pH 7.4 and 30°C | Shewanella oneidensis |