Literature summary for 1.7.1.17 extracted from

Fazeliyan, E.; Sadeghi, M.; Forouzandeh, S.; Doosti, A.; Mohammadi Moghadam, F.; Sedehi, M.; Emadi, Z.; Sadeghi, R.
Decolorization mechanism, identification of an FMN-dependent NADH-azoreductase from a moderately halotolerant Staphylococcus sp. MEH038S, and toxicity assessment of biotransformed metabolites (2021), Water Environ. Res., 93, 2072-2083 .

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Application

Application	Comment	Organism
additional information	the enzyme can be applied for textile wastewater treatment	Staphylococcus sp. MEH038S

General Stability

General Stability	Organism
more than 90% decolorization efficiency is observed under high-salt conditions (5-40 g/l NaCl)	Staphylococcus sp. MEH038S

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+	Staphylococcus sp. MEH038S	-	2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus sp. MEH038S	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+	-	Staphylococcus sp. MEH038S	2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+	-	?
Reactive black 5 + 2 NADH + 2 H+	-	Staphylococcus sp. MEH038S	n-hydroxy-benzenamine + dimethyl phthalate + phenylethyl alcohol + 4-methylphenol + 3-(methylthio)propanoic acid methyl ester + 2 NAD+	-	?

Synonyms

Synonyms	Comment	Organism
AzoR	-	Staphylococcus sp. MEH038S
FMN-dependent NADH-azoreductase	-	Staphylococcus sp. MEH038S

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Staphylococcus sp. MEH038S

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Staphylococcus sp. MEH038S

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Staphylococcus sp. MEH038S
NADH	-	Staphylococcus sp. MEH038S

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Release 2023.1 (January 2023)