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Literature summary for 1.7.1.2 extracted from
- Purification and properties of the NAD(P)H:nitrate reductase of the yeast Rhodotorula glutinis (1977), Biochim. Biophys. Acta, 482, 272-285.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | - |
Rhodotorula glutinis |  |
| Cyanate | - |
Rhodotorula glutinis | |
| cyanide | - |
Rhodotorula glutinis | |
| nitrite | - |
Rhodotorula glutinis | |
| p-chloromercuribenzoate | - |
Rhodotorula glutinis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
NADPH | - |
Rhodotorula glutinis | |
| 0.125 | - |
nitrate | cosubstrate NADPH | Rhodotorula glutinis | |
| 0.16 | - |
NADH | - |
Rhodotorula glutinis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | molybdoprotein | Rhodotorula glutinis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 118000 | - |
2 * 118000, Rhodotorula glutinis, SDS-PAGE | Rhodotorula glutinis |
| 230000 | - |
Rhodotorula glutinis, sucrose density gradient centrifugation, gel filtration | Rhodotorula glutinis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodotorula glutinis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 148.2 | - |
- |
Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD(P)H + nitrate | also reduction of ClO3-, K3Fe(CN)6, cytochrome c and 2,6-dichlorophenolindophenol with NADPH or NADH | Rhodotorula glutinis | NAD(P)+ + nitrite + H2O | - |
? | |
| NAD(P)H + nitrate | reduced methyl viologen | Rhodotorula glutinis | NAD(P)+ + nitrite + H2O | - |
? | |
| NAD(P)H + nitrate | other electron donors: combination of dithionite with flavin nucleotides or viologens | Rhodotorula glutinis | NAD(P)+ + nitrite + H2O | - |
? | |
| nitrate + NADH + H+ | - |
Rhodotorula glutinis | nitrite + NAD+ + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 118000, Rhodotorula glutinis, SDS-PAGE | Rhodotorula glutinis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Rhodotorula glutinis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b557 | contains cytochrome b557 | Rhodotorula glutinis | |
| FAD | complete reduction of cytochrome by NADPH takes place only when added FAD is present in the enzyme preparation before addition of NADPH | Rhodotorula glutinis | |
| NADH | at low nitrate concentration: more active with NADH | Rhodotorula glutinis | |
| NADH | at high nitrate concentration: more active with NADPH | Rhodotorula glutinis | |
| NADPH | at low nitrate concentration: more active with NADH | Rhodotorula glutinis | |
| NADPH | at high nitrate concentration: more active with NADPH | Rhodotorula glutinis | |
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