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Literature summary for 1.7.1.2 extracted from
- Isolation, purification, and characterization of nitrate reductase from a salt-tolerant Rhodotorula glutinis yeast strain grown in the presence of tungsten (2005), Biochemistry (Moscow), 70, 809-814.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | at low concentration | Rhodotorula glutinis |  |
| cyanide | at low concentration | Rhodotorula glutinis | |
| additional information | insensitive to 1 mM tungsten | Rhodotorula glutinis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.74 | - |
nitrate | apparent KM | Rhodotorula glutinis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | contains 0.177 mol of Mo per mol | Rhodotorula glutinis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, SDS-PAGE | Rhodotorula glutinis |
| 130000 | - |
SDS-PAGE | Rhodotorula glutinis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Toyopearl 650M column chromatography, Sephacryl S-200 gel filtration and DEAE-cellulose DE-52 column chromatography | Rhodotorula glutinis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
after purification | Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NAD(P)H | - |
Rhodotorula glutinis | nitrite + NAD(P)+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 65000, SDS-PAGE | Rhodotorula glutinis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD(P)H:nitrate-oxidoreductase | - |
Rhodotorula glutinis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | - |
Rhodotorula glutinis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rhodotorula glutinis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Rhodotorula glutinis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)H | - |
Rhodotorula glutinis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0094 | - |
cyanide | at low concentration | Rhodotorula glutinis | |
| 0.044 | - |
azide | at low concentration | Rhodotorula glutinis | |
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