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Literature summary for 1.7.1.3 extracted from

  • Savidov, N.A.; Alikulov, Z.A.; Lips, S.H.
    Identification of an endogenous NADPH-regenerating system coupled to nitrate reduction in vitro in plant and fungal crude extracts (1998), Plant Sci., 133, 33-45.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
NADPH elevated concentrations Hordeum vulgare
NADPH elevated concentrations Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum
-
Neurospora crassa
Molybdenum
-
Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADPH Neurospora crassa key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro nitrite + NADP+ + H2O
-
?
nitrate + NADPH Hordeum vulgare key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro nitrite + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
L. cv. Steptoe nar1a and nar1a
-
Hordeum vulgare
-
nar7w genotypes
-
Neurospora crassa
-
nit-1 mutant
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Neurospora crassa
-

Storage Stability

Storage Stability Organism
-80┬░C, extract, 6 months, without loss of activity Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADPH
-
Neurospora crassa nitrite + NADP+ + H2O
-
?
nitrate + NADPH
-
Hordeum vulgare nitrite + NADP+ + H2O
-
?
nitrate + NADPH key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro Neurospora crassa nitrite + NADP+ + H2O
-
?
nitrate + NADPH key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro Hordeum vulgare nitrite + NADP+ + H2O
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
28
-
assay at Neurospora crassa
28
-
assay at Hordeum vulgare

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
10
-
quite stable for at least 24 h Neurospora crassa
10
-
quite stable for at least 24 h Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Neurospora crassa
7.7
-
assay at Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
FAD
-
Neurospora crassa
FAD
-
Hordeum vulgare