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Literature summary for extracted from

  • Antoine, A.D.
    Purification and properties of the nitrate reductase isolated from Neurospora crassa mutant nit-3. Kinetics, molecular weight determination, and cytochrome involvement (1974), Biochemistry, 13, 2289-2294.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information nit-3 mutant (FGSC 262): reduced FAD-nitrate reductase and reduced methylviologen-nitrate reductase activities Neurospora crassa


Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate at 0.1 mM complete inhibition Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
sucrose density gradient centrifugation, gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADPH Neurospora crassa
nitrite + NADP+ + H2O


Organism UniProt Comment Textmining
Neurospora crassa
wild-type STA4, FGSC 358 and nit-1 mutant, FGSC 262

Purification (Commentary)

Purification (Comment) Organism
partially purified Neurospora crassa


Reaction Comment Organism Reaction ID
nitrite + NADP+ + H2O = nitrate + NADPH + H+ sulfhydryl groups may participate in the binding of the protein subunits Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
Neurospora crassa

Storage Stability

Storage Stability Organism
-20┬░C, stable for 6 months or longer, 5% loss of activity Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADPH
Neurospora crassa nitrite + NADP+ + H2O
nitrate + NADPH associated FAD-nitrate reductase and methylviologen-nitrate activity Neurospora crassa nitrite + NADP+ + H2O


Subunits Comment Organism
More at least 2 subunits suggested Neurospora crassa

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
labile, loss of acitvity after 2 min Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
optima of associated activities Neurospora crassa


Cofactor Comment Organism Structure
cytochrome b557
Neurospora crassa
Neurospora crassa