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Literature summary for 1.7.1.3 extracted from

  • Padidam , M.; Venkateswarlu, K.; Johri, M.M.
    Ammonium represses NADPH-nitrate reductase in the moss Funaria hygrometrica (1991), Plant Sci., 75, 185-194.
No PubMed abstract available

General Stability

General Stability Organism
loss of activity with ammonium chloride or ammonium nitrate at 10 mM to growing cells, faster decay in washed and resuspended cells Funaria hygrometrica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012 0.015 nitrate
-
Funaria hygrometrica

Metals/Ions

Metals/Ions Comment Organism Structure
NH4+ represses the intracellular NO3- assimilation, which is required for the induction and maintenace of NADPH nitrate reductase, enzyme is regulated by NH4+ at the transcriptional level Funaria hygrometrica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
4 * 116000 Funaria hygrometrica
450000
-
native gel electrophoresis Funaria hygrometrica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADPH Funaria hygrometrica
-
nitrite + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Funaria hygrometrica
-
Hedw.
-

Source Tissue

Source Tissue Comment Organism Textmining
chloronema
-
Funaria hygrometrica
-

Storage Stability

Storage Stability Organism
half life of about 2 h with ammonium sulfate or ammonium chloride as sole N-source Funaria hygrometrica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADPH
-
Funaria hygrometrica nitrite + NADP+ + H2O
-
?
nitrate + NADPH associated cytochrome c reductase activity Funaria hygrometrica nitrite + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 116000 Funaria hygrometrica

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Funaria hygrometrica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Funaria hygrometrica