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Literature summary for 1.7.1.3 extracted from
- Identification of an endogenous NADPH-regenerating system coupled to nitrate reduction in vitro in plant and fungal crude extracts (1998), Plant Sci., 133, 33-45.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | elevated concentrations | Hordeum vulgare |  |
| NADPH | elevated concentrations | Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | - |
Neurospora crassa | |
| Molybdenum | - |
Hordeum vulgare | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | Neurospora crassa | key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | Hordeum vulgare | key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro | nitrite + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
L. cv. Steptoe nar1a and nar1a | - |
| Hordeum vulgare | - |
nar7w genotypes | - |
| Neurospora crassa | - |
nit-1 mutant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Neurospora crassa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, extract, 6 months, without loss of activity | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | - |
Hordeum vulgare | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro | Hordeum vulgare | nitrite + NADP+ + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Neurospora crassa |
| 28 | - |
assay at | Hordeum vulgare |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | - |
quite stable for at least 24 h | Neurospora crassa |
| 10 | - |
quite stable for at least 24 h | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Neurospora crassa |
| 7.7 | - |
assay at | Hordeum vulgare |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Neurospora crassa | |
| FAD | - |
Hordeum vulgare | |
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