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Literature summary for 1.7.1.3 extracted from

  • Horner, R.D.
    Purification and comparison of nit-1 and wild-type NADPH:nitrate reductases of Neurospora crassa (1983), Biochim. Biophys. Acta, 744, 7-15.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information nit-1 mutant, suggested to produce the complete apoenzyme Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022 0.05 NADPH
-
Neurospora crassa
0.29
-
nitrate
-
Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum
-
Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
monodimer, 2 * 145000 Neurospora crassa
290000
-
-
Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADPH Neurospora crassa
-
nitrite + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate and ammonium sulfate precipitation, hemoglobin-Sepharose column, Bio-Gel A, FAD-Sepharose affinity column. proteolysis during extensive purification Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.063
-
nit-1 mutant Neurospora crassa
1.24
-
wild-type Neurospora crassa

Storage Stability

Storage Stability Organism
-10┬░C, 0.1 M sodium phosphate buffer, pH 7.3, 0.17 M NaCl, 1 mM dithiothreitol, 5 m M EDTA, 0.5 mM PMSF, 1%ethanol, 30% glycerol Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADPH
-
Neurospora crassa nitrite + NADP+ + H2O
-
?
nitrate + NADPH 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase Neurospora crassa nitrite + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
dimer monodimer, 2 * 145000 Neurospora crassa
More nit-1 mutant enzyme is the apoprotein of nitrate reductase Neurospora crassa

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
-
Neurospora crassa

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
49
-
50% loss of activity after 1.5 min Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Neurospora crassa