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Literature summary for 1.7.1.3 extracted from

  • Polcyn, W.; Lucinski, R.
    Aerobic and anaerobic nitrate and nitrite reduction in free-living cells of Bradyrhizobium sp. (Lupinus) (2003), FEMS Microbiol. Lett., 226, 331-337.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information growth stimulation is higher on nitrate than on nitrite Bradyrhizobium sp.
NH4+ slight activation of the enzyme under anaerobic conditions Bradyrhizobium sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information intracellularly accumulated nitrite can inhibit nitrate uptake, addition of nitrite at concentrations above 5 mM is toxic and causes growth retardation Bradyrhizobium sp.
NH4+ inhibits the enzyme under aerobic conditions Bradyrhizobium sp.

Organism

Organism UniProt Comment Textmining
Bradyrhizobium sp.
-
Lupinus, strain USDA 3045
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture free-living cells, yeast extract/mannitol medium Bradyrhizobium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.02
-
activity under aerobic conditions in presence of NH4+ Bradyrhizobium sp.
0.1
-
activity under aerobic conditions Bradyrhizobium sp.
0.3
-
activity under anaerobic conditions Bradyrhizobium sp.
0.37
-
activity under anaerobic conditions in presence of NH4+ Bradyrhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + reduced benzyl viologen
-
Bradyrhizobium sp. nitrite + benzyl viologen
-
?

Synonyms

Synonyms Comment Organism
nitrate reductase
-
Bradyrhizobium sp.
NR
-
Bradyrhizobium sp.

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
additional information
-
a growth temperature above 20┬░C is required Bradyrhizobium sp.
30
-
assay at Bradyrhizobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Bradyrhizobium sp.

Cofactor

Cofactor Comment Organism Structure
benzyl viologen
-
Bradyrhizobium sp.