Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is nitrate-inducible | Tuber borchii |
Cloned (Comment) | Organism |
---|---|
gene tbnr1, DNA and amino acid sequence determination and analysis, expression analysis | Tuber borchii |
gene tpnr1, DNA sequence determination and analysis, construction of a cDNA library | Tilia platyphyllos |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | Tilia platyphyllos | enzyme is involved in nitrate assimilation | nitrite + NADP+ | - |
? | |
nitrate + NADPH | Tuber borchii | enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis | nitrite + NADP+ | - |
? | |
nitrate + NADPH | Tuber borchii Z43 | enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis | nitrite + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tilia platyphyllos | Q8H1T7 | gene tpnr1 | - |
Tuber borchii | Q8J259 | gene tbnr1, ascomycete | - |
Tuber borchii Z43 | Q8J259 | gene tbnr1, ascomycete | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ectomycorrhiza | symbiosis with Tilia platyphyllos, root | Tuber borchii | - |
ectomycorrhiza | symbiosis with Tuber borchii, mycelium | Tilia platyphyllos | - |
additional information | the enzymatic activity is increased in root ectomycchorizae in symbiosis with the fungus Tuber borchii, while the expression of the endogenous enzyme is decreased | Tilia platyphyllos | - |
additional information | the enzyme expression and activity is increased in ectomycchorizae in symbiosis with Tilia platyphyllos | Tuber borchii | - |
mycelium | - |
Tuber borchii | - |
root | non-inoculated | Tilia platyphyllos | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0014 | - |
root | Tilia platyphyllos |
0.003 | - |
ectomycchorizae | Tilia platyphyllos |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | - |
Tuber borchii | nitrite + NADP+ | - |
? | |
nitrate + NADPH | - |
Tilia platyphyllos | nitrite + NADP+ | - |
? | |
nitrate + NADPH | enzyme is involved in nitrate assimilation | Tilia platyphyllos | nitrite + NADP+ | - |
? | |
nitrate + NADPH | enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis | Tuber borchii | nitrite + NADP+ | - |
? | |
nitrate + NADPH | - |
Tuber borchii Z43 | nitrite + NADP+ | - |
? | |
nitrate + NADPH | enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis | Tuber borchii Z43 | nitrite + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nitrate reductase | - |
Tuber borchii |
nitrate reductase | - |
Tilia platyphyllos |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Tuber borchii |
7.4 | - |
assay at | Tilia platyphyllos |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme amino acid sequence contains a molybdate-cofactor, a cytochrome b5 heme, and a FAD binding domain | Tuber borchii | |
NADPH | dependent on | Tuber borchii | |
NADPH | dependent on | Tilia platyphyllos |