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Literature summary for 1.7.1.3 extracted from

  • Guescini, M.; Pierleoni, R.; Palma, F.; Zeppa, S.; Vallorani, L.; Potenza, L.; Sacconi, C.; Giomaro, G.; Stocchi, V.
    Characterization of the Tuber borchii nitrate reductase gene and its role in ectomycorrhizae (2003), Mol. Genet. Genomics, 269, 807-816.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is nitrate-inducible Tuber borchii

Cloned(Commentary)

Cloned (Comment) Organism
gene tbnr1, DNA and amino acid sequence determination and analysis, expression analysis Tuber borchii
gene tpnr1, DNA sequence determination and analysis, construction of a cDNA library Tilia platyphyllos

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADPH Tilia platyphyllos enzyme is involved in nitrate assimilation nitrite + NADP+
-
?
nitrate + NADPH Tuber borchii enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis nitrite + NADP+
-
?
nitrate + NADPH Tuber borchii Z43 enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis nitrite + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Tilia platyphyllos Q8H1T7 gene tpnr1
-
Tuber borchii Q8J259 gene tbnr1, ascomycete
-
Tuber borchii Z43 Q8J259 gene tbnr1, ascomycete
-

Source Tissue

Source Tissue Comment Organism Textmining
ectomycorrhiza symbiosis with Tilia platyphyllos, root Tuber borchii
-
ectomycorrhiza symbiosis with Tuber borchii, mycelium Tilia platyphyllos
-
additional information the enzymatic activity is increased in root ectomycchorizae in symbiosis with the fungus Tuber borchii, while the expression of the endogenous enzyme is decreased Tilia platyphyllos
-
additional information the enzyme expression and activity is increased in ectomycchorizae in symbiosis with Tilia platyphyllos Tuber borchii
-
mycelium
-
Tuber borchii
-
root non-inoculated Tilia platyphyllos
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.0014
-
root Tilia platyphyllos
0.003
-
ectomycchorizae Tilia platyphyllos

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADPH
-
Tuber borchii nitrite + NADP+
-
?
nitrate + NADPH
-
Tilia platyphyllos nitrite + NADP+
-
?
nitrate + NADPH enzyme is involved in nitrate assimilation Tilia platyphyllos nitrite + NADP+
-
?
nitrate + NADPH enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis Tuber borchii nitrite + NADP+
-
?
nitrate + NADPH
-
Tuber borchii Z43 nitrite + NADP+
-
?
nitrate + NADPH enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis Tuber borchii Z43 nitrite + NADP+
-
?

Synonyms

Synonyms Comment Organism
nitrate reductase
-
Tuber borchii
nitrate reductase
-
Tilia platyphyllos

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Tuber borchii
7.4
-
assay at Tilia platyphyllos

Cofactor

Cofactor Comment Organism Structure
additional information enzyme amino acid sequence contains a molybdate-cofactor, a cytochrome b5 heme, and a FAD binding domain Tuber borchii
NADPH dependent on Tuber borchii
NADPH dependent on Tilia platyphyllos