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Literature summary for 1.7.1.4 extracted from

  • Prodouz, K.N.; Garrett, R.H.
    Neurospora crassa NAD(P)H-nitrite reductase. Studies on its composition and structure (1981), J. Biol. Chem., 256, 9711-9717.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
 FAD
-
 Neurospora crassa
0.0075
-
 NO2-
-
 Neurospora crassa
0.015
-
 NADPH
-
 Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 9-10 mol Fe per mol of enzyme, heme iron associated with siroheme moieties, nonheme iron involved in iron-sulfur centers Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 2 * 140000, SDS-PAGE Neurospora crassa
289400
-
 deduced from amino acid composition Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD(P)H + nitrite + H+ Neurospora crassa
-
 NAD(P)+ + NH3 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + nitrite + H+
-
 Neurospora crassa NAD(P)+ + NH3 + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 140000, SDS-PAGE Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FAD exogenously added FAD is required for activity in vitro Neurospora crassa
flavin little or no flavin associated with purified protein Neurospora crassa
NADPH
-
 Neurospora crassa
siroheme
-
 Neurospora crassa