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Literature summary for 1.7.1.6 extracted from
- Purification and some properties of new coccine (NC)-reductase from Bacillus cereus T-105 strain (1977), Agric. Biol. Chem., 41, 2323-2329.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FMN | increases activity | Bacillus cereus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 92% inhibition | Bacillus cereus | |
| Cu2+ | 1 mM, 88% inhibition | Bacillus cereus | |
| Fe2+ | 1 mM, 90% inhibition | Bacillus cereus | |
| Fe3+ | 1 mM, 85% inhibition | Bacillus cereus | |
| Hg2+ | 1 mM, 75% inhibition | Bacillus cereus | |
| additional information | not inhibited by N-ethylmaleimide, p-chloromercuribenzoate and monoiodoacetic acid | Bacillus cereus | |
| SDS | 1 mM, 50% inhibition | Bacillus cereus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Bacillus cereus | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight activation | Bacillus cereus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
T-105 | - |
| Bacillus cereus T-105 | - |
T-105 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus cereus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.566 | - |
- |
Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-(dimethylamino)azobenzene + 2 NADPH | - |
Bacillus cereus | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
| 4-(dimethylamino)azobenzene + 2 NADPH | - |
Bacillus cereus T-105 | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
| new coccine + NADPH | - |
Bacillus cereus | ? | - |
? | |
| new coccine + NADPH | - |
Bacillus cereus T-105 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 70 | at 5°C and 70°C: about 55% of maximal activity | Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, stable up to | Bacillus cereus |
| 80 | - |
10 min, 45% loss of activity | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | pH 4.0: about 10% activity, pH 10.0: about 20% activity | Bacillus cereus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 5°C, 7 days, stable | Bacillus cereus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | increases activity | Bacillus cereus | |
| flavin | prosthetic group seems to be flavin | Bacillus cereus | |
| additional information | no activity with NADH | Bacillus cereus | |
| NADPH | specific for | Bacillus cereus | |
| riboflavin | no activation | Bacillus cereus | |
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