Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Orange II | substrate inhibition at high concentration | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
1-phenylazo-2-hydroxy-6-sulfonaphthalene | - |
Pseudomonas sp. | |
0.001 | - |
1-(4'-sulfophenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.0013 | - |
1-(2'-methyl-4'-sulfophenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.0014 | - |
1-phenylazo-2-hydroxy-6-carboxynaphthalene | - |
Pseudomonas sp. | |
0.0015 | - |
1-(3'-carboxyphenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.0018 | - |
1-(3'-sulfophenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.0018 | - |
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene | - |
Pseudomonas sp. | |
0.005 | - |
NADPH | - |
Pseudomonas sp. | |
0.0052 | - |
1-(4'-phosphophenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.008 | - |
1-(4'-carboxyphenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.0105 | - |
1-(3'-phosphophenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.013 | - |
1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene | - |
Pseudomonas sp. | |
0.0132 | - |
1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene | - |
Pseudomonas sp. | |
0.0137 | - |
1-(2'-sulfophenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.0148 | - |
1-(4'-sulfoaminophenylazo)-2-naphthol | - |
Pseudomonas sp. | |
0.017 | - |
1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene | - |
Pseudomonas sp. | |
0.0198 | - |
1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene | - |
Pseudomonas sp. | |
0.18 | - |
NADH | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, strain KF46, SDS-PAGE, Orange II azoreductase | Pseudomonas sp. |
32000 | - |
strain KF 46, gel filtration, Orange II azoreductase | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
Orange II azoreductase induced by both Orange II and carboxy-Orange II | - |
Pseudomonas sp. KF46 | - |
Orange II azoreductase induced by both Orange II and carboxy-Orange II | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2868 | - |
orange I azoreductase, strain KF24 | Pseudomonas sp. |
17830 | - |
orange II azoreductase, strain KF46 | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, several months | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2'-methyl-4'-sulfophenylazo)-2-naphthol + NADH + H+ | - |
Pseudomonas sp. | 4-amino-2-methyl-1-benzenesulfonic acid + NAD+ + 1-amino-2-hydroxynaphthalene | - |
r | |
1-(2'-methyl-4'-sulfophenylazo)2-naphthol + NAD(P)H | - |
Pseudomonas sp. | 4-amino-2-methyl-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(2'-sulfophenylazo)-2-naphthol + NADH + H+ | - |
Pseudomonas sp. | ? | - |
r | |
1-(3'-carboxyphenylazo)-2-naphthol + NAD(P)H | - |
Pseudomonas sp. | 3-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(3'-phosphophenylazo)-2-naphthol + NAD(P)H | - |
Pseudomonas sp. | 3-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(3'-sulfophenylazo)-2-naphthol + NAD(P)H | - |
Pseudomonas sp. | 3-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene + NADH + H+ | - |
Pseudomonas sp. | ? | - |
r | |
1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H | - |
Pseudomonas sp. | 4-carboxyphenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | - |
Pseudomonas sp. | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | - |
Pseudomonas sp. KF46 | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-naphthol + NADH + H+ | i.e. carboxy-Orange II | Pseudomonas sp. | 4-aminobenzoate + NAD+ + ? | - |
r | |
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H | - |
Pseudomonas sp. | 4-nitrophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene | - |
? | |
1-(4'-phosphophenylazo)-2-naphthol + NAD(P)H | - |
Pseudomonas sp. | 4-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-phosphophenylazo)-2-naphthol + NAD(P)H | - |
Pseudomonas sp. KF46 | 4-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-sulfonaminophenylazo-)2-naphthol + NAD(P)H | - |
Pseudomonas sp. | 4-amino-1-benzenesulfonamine + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-sulfonaminophenylazo-)2-naphthol + NAD(P)H | - |
Pseudomonas sp. KF46 | 4-amino-1-benzenesulfonamine + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene + NAD(P)H | - |
Pseudomonas sp. | 4-sulfophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-carboxynaphthalene | - |
? | |
1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H | - |
Pseudomonas sp. | 4-sulfophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene | - |
? | |
1-phenylazo-2-hydroxy-6-carboxynaphthalene + NAD(P)H | - |
Pseudomonas sp. | phenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-carboxynaphthalene | - |
? | |
1-phenylazo-2-hydroxy-6-sulfonaphthalene + NAD(P)H | - |
Pseudomonas sp. | phenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | Orange II azoreductase: requirement for substrates with a 2-naphthol moiety | Pseudomonas sp. | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | Orange II azoreductase: requirement for substrates with a 2-naphthol moiety | Pseudomonas sp. KF46 | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H | trivial name Orange II | Pseudomonas sp. | 4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H | trivial name Orange II | Pseudomonas sp. KF46 | 4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
Solar Orange + NADPH | - |
Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, strain KF46, SDS-PAGE, Orange II azoreductase | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20 C: about 25% activity with carboxy-Orange II, about 30% activity with Orange II, 50 C: about 95% activity with carboxy-Orange II, about 80% activity with Orange II | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable below | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | Orange II + NADH | Pseudomonas sp. |
6.5 | - |
carboxy-Orange II + NADH | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas sp. | |
NADPH | - |
Pseudomonas sp. |