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Literature summary for 1.7.1.6 extracted from

  • Bluemel, S.; Stolz, A.
    Cloning and characterization of the gene coding for the aerobic azoreductase from Pigmentiphaga kullae K24 (2003), Appl. Microbiol. Biotechnol., 62, 186-190.
    View publication on PubMed
Search for more literature for 1.7.1.6

Application

Application Comment Organism
environmental protection potential for the treatment of azo dye contaminated wastewater Pigmentiphaga kullae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS using a phage T7-promotor system Pigmentiphaga kullae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 SDS-PAGE Pigmentiphaga kullae
20560
-
 calculated from the deduced amino acid sequence Pigmentiphaga kullae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H Pigmentiphaga kullae substrate can be utilized as sole carbon and energy source 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H Pigmentiphaga kullae K24 substrate can be utilized as sole carbon and energy source 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene
-
 ?

Organism

Organism UniProt Comment Textmining
Pigmentiphaga kullae Q6YAN1 previously Pseudomonas sp. K24
-
Pigmentiphaga kullae K24 Q6YAN1 previously Pseudomonas sp. K24
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pigmentiphaga kullae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 purified enzyme, pH 7.1 with 1-(4'-carboxyphenylazo)-4-naphthol and NADH as substrates Pigmentiphaga kullae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H substrate can be utilized as sole carbon and energy source Pigmentiphaga kullae 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H substrate is carboxy-Orange I Pigmentiphaga kullae 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H substrate can be utilized as sole carbon and energy source Pigmentiphaga kullae K24 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H substrate is carboxy-Orange I Pigmentiphaga kullae K24 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene
-
 ?
4-(4-nitrophenylazo)-1-naphthol + NAD(P)H substrate is Magneson II, 13% of the activity with carboxy-Orange I Pigmentiphaga kullae ?
-
 ?
4-(4-nitrophenylazo)-1-naphthol + NAD(P)H substrate is Magneson II, 13% of the activity with carboxy-Orange I Pigmentiphaga kullae K24 ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pigmentiphaga kullae
NADPH
-
 Pigmentiphaga kullae