Application | Comment | Organism |
---|---|---|
industry | industrial use of the enzyme in the biodegradation process of azo compounds | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
into the vector pET-22b for expression in Escherichia coli BL21DE3 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the structures of oxidized and reduced AzoR, and in complex with the inhibitor 3,3'-methylene-bis(4-hydroxycoumarin) are determined at resolutions from 1.4 to 2.3 A | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F162A | Phe-162 is chosen because it is predicted to participate in the substrate binding on top of the isoalloxazine ring, as observed in the AzoR-inhibitor structure | Escherichia coli |
R59A | the results indicate that Arg-59 decides the substrate specificity of AzoR | Escherichia coli |
Y120A | Tyr-120 is chosen because it is predicted to participate in the substrate binding on top of the isoalloxazine ring, as observed in the AzoR-inhibitor structure | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3'-methylene-bis(4-hydroxycoumarin) | dicoumarol | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
4'-dimethylaminoazobenzene-2-carboxylic acid | mutant Y120A | Escherichia coli | |
0.0419 | - |
4'-dimethylaminoazobenzene-2-carboxylic acid | wild-type enzyme | Escherichia coli | |
0.0706 | - |
NADH | wild-type enzyme | Escherichia coli | |
0.0849 | - |
4'-dimethylaminoazobenzene-2-carboxylic acid | mutant R59A | Escherichia coli | |
0.1006 | - |
p-methyl red | mutant R59A | Escherichia coli | |
0.101 | - |
4'-dimethylaminoazobenzene-2-carboxylic acid | mutant F162A | Escherichia coli | |
0.23 | - |
p-methyl red | mutant Y120A | Escherichia coli | |
0.266 | - |
p-methyl red | wild-type enzyme | Escherichia coli | |
0.403 | - |
NADH | mutant R59A | Escherichia coli | |
0.637 | - |
NADH | mutant Y120A | Escherichia coli | |
0.739 | - |
NADH | mutant F162A | Escherichia coli | |
0.867 | - |
p-methyl red | mutant F162A | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
- |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P41407 | - |
- |
Purification (Comment) | Organism |
---|---|
His-tagged proteins are purified by gravity-flow chromatography using nickel-nitrilotriacetic acid-agarose | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-1,4-naphthoquinone + NADH + H+ | menadione, vitamin K3 | Escherichia coli | ? | - |
? | |
4'-diethylaminoazobenzene-2-carboxylic acid + NADH + H+ | ethyl red | Escherichia coli | ? | - |
? | |
4'-dimethylaminoazobenzene-2-carboxylic acid + NADH + H+ | methyl red | Escherichia coli | 2-aminobenzoic acid + N,N'-dimethyl-p-phenylenediamine + NAD+ | - |
? | |
p-methyl red + NADH + H+ | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 23000 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AzoR | - |
Escherichia coli |
azoreductase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli | |
NADH | - |
Escherichia coli |