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show all sequences of 1.7.1.6

Cloning and characterization of a robust recombinant azoreductase from Shewanella xiamenensis BC01

Zhang, X.; Ng, I.; Chang, J.; J. Taiwan Inst. Chem. Eng. 61, 97-105 (2015)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene azoR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) from three different vectors pET32a, pET28a and pET20bI. pET32a contains an N-terminal Trx-tag encoding for a 109-amino-acid highly soluble polypeptide thioredoxin, TrxA, the pET28a is an original vector without special fusion protein, while pET20bI (similar to pET22b) contains an N-terminal pelB signal sequence for potential periplasmic localization resulting in recombinant enzymes of molecular weight of 40.4 kDa, 26.2 kDa and 25.8 kDa, respectively. Recombinant AzrS_32 is soluble by 98%, AzrS_28 by 90%, while AzrS_20 is mostly accumulated as inclusion bodies, that have no activity, although pelB leads the target protein to the periplasmic space, where there is a more favorable environment for folding and disulfide bond formation. By contrast, the protein amount of AzrS_28 (i.e. 278 mg/l) is slightly higher than that of AzrS_32 (i.e. 253 mg/l). Therefore, the plasmid pET28a is considered as the ideal vector for production of AzrS
Shewanella xiamenensis
General Stability
General Stability
Organism
AzrS is an oxygen-insensitive, FMN-dependent, robust against to organic solvent and has extremely high and stable activity at room temperature
Shewanella xiamenensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
loss of 30.6% activity at 10% v/v
Shewanella xiamenensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten steady-state kinetics
Shewanella xiamenensis
0.1056
-
Methyl orange
recombinant enzyme, pH 6.5, 22C
Shewanella xiamenensis
0.1593
-
Methyl red
recombinant enzyme, pH 6.5, 22C
Shewanella xiamenensis
0.2792
-
Congo red
recombinant enzyme, pH 6.5, 22C
Shewanella xiamenensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates at 5 mM
Shewanella xiamenensis
Co2+
activates at 5 mM
Shewanella xiamenensis
Cr3+
activates at 5 mM
Shewanella xiamenensis
Cu2+
activates at 5 mM
Shewanella xiamenensis
Fe3+
activates at 5 mM
Shewanella xiamenensis
K+
activates at 5 mM
Shewanella xiamenensis
Mg2+
41% activation at 5 mM, best activating cation
Shewanella xiamenensis
Mn2+
activates at 5 mM
Shewanella xiamenensis
Na+
activates at 5 mM
Shewanella xiamenensis
Zn2+
activates at 5 mM
Shewanella xiamenensis
Organic Solvent Stability
Organic Solvent
Commentary
Organism
1-butanol
loss of 85.4% activity at 10% v/v
Shewanella xiamenensis
1-propanol
loss of 40.9% activity at 10% v/v
Shewanella xiamenensis
Acetone
at 10% v/v, the enzyme is completely stable
Shewanella xiamenensis
chloroform
loss of 55.6% activity at 10% v/v
Shewanella xiamenensis
DMSO
loss of 12.2% activity at 10% v/v
Shewanella xiamenensis
Ethanol
loss of 7.6% activity at 10% v/v
Shewanella xiamenensis
isobutanol
loss of 73.8% activity at 10% v/v
Shewanella xiamenensis
isopropanol
gain of 5.8% activity at 10% v/v
Shewanella xiamenensis
Methanol
at 10% v/v, the enzyme is completely stable
Shewanella xiamenensis
additional information
AzrS is an oxygen-insensitive, FMN-dependent, robust against to organic solvent and has extremely high and stable activity at room temperature
Shewanella xiamenensis
N,N-dimethylformamide
loss of 3.0% activity at 10% v/v
Shewanella xiamenensis
tert-Butanol
loss of 19.0% activity at 10% v/v
Shewanella xiamenensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Shewanella xiamenensis
A0A073KHN9
-
-
Shewanella xiamenensis BC01
A0A073KHN9
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Shewanella xiamenensis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
254
-
purified recombinant enzyme, pH 6.5, 22C, substrate congo red
Shewanella xiamenensis
5152
-
purified recombinant enzyme, pH 6.5, 22C, substrate methl orange
Shewanella xiamenensis
97153
-
purified recombinant enzyme, pH 6.5, 22C, substrate methl red
Shewanella xiamenensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Congo red + NADH + H+
-
743182
Shewanella xiamenensis
? + NAD+
-
-
-
?
Congo red + NADH + H+
-
743182
Shewanella xiamenensis BC01
? + NAD+
-
-
-
?
methyl orange + NADH + H+
-
743182
Shewanella xiamenensis
? + NAD+
-
-
-
?
methyl orange + NADH + H+
-
743182
Shewanella xiamenensis BC01
? + NAD+
-
-
-
?
methyl red + NADH + H+
i.e. 4-dimethylaminoazobenzene-2'-carboxylic acid
743182
Shewanella xiamenensis
anthranilate + N,N'-dimethylaminoaniline + NAD+
-
-
-
?
methyl red + NADH + H+
i.e. 4-dimethylaminoazobenzene-2'-carboxylic acid
743182
Shewanella xiamenensis BC01
anthranilate + N,N'-dimethylaminoaniline + NAD+
-
-
-
?
additional information
substrate specificity: the catalytic efficiency of AzrS follows the descending order of methyl red, methyl orange, and congo red. The enzyme has a wide substrate range of azo dyes
743182
Shewanella xiamenensis
?
-
-
-
-
additional information
substrate specificity: the catalytic efficiency of AzrS follows the descending order of methyl red, methyl orange, and congo red. The enzyme has a wide substrate range of azo dyes
743182
Shewanella xiamenensis BC01
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 21300, about, sequence calculation, x * 40400, recombinant AzrS_32 with TrxA, DS-PAGE, x * 26200, recombinant untagged AzrS_28, SDS-PAGE, x * 25800, recombinant AzrS_20 with pelB signal peptide
Shewanella xiamenensis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
30
-
Shewanella xiamenensis
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
50
activity range, profile overview
Shewanella xiamenensis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
10
50
most stable at 20C, loss of activity at 60C
Shewanella xiamenensis
22
-
AzrS is an oxygen-insensitive, FMN-dependent, robust against to organic solvent and has extremely high and stable activity at room temperature
Shewanella xiamenensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Shewanella xiamenensis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
activity range, profile overview
Shewanella xiamenensis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
most stable at pH 7.0-8.0, loss of activity at pH 5.0 and below
Shewanella xiamenensis
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
dependent on, best at 0.125 mM
Shewanella xiamenensis
additional information
AzrS shows activity with both NADH and NADPH. The maximum relative activity of AzrS with 0.875 mM NADPH or NADH is 28.1% and 100%, respectively, indicating that AzrS shows a preference for NADH rather than NADPH
Shewanella xiamenensis
NADH
preferred cofactor
Shewanella xiamenensis
NADPH
low activity
Shewanella xiamenensis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Shewanella xiamenensis
sequence calculation
-
5.23
Cloned(Commentary) (protein specific)
Commentary
Organism
gene azoR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) from three different vectors pET32a, pET28a and pET20bI. pET32a contains an N-terminal Trx-tag encoding for a 109-amino-acid highly soluble polypeptide thioredoxin, TrxA, the pET28a is an original vector without special fusion protein, while pET20bI (similar to pET22b) contains an N-terminal pelB signal sequence for potential periplasmic localization resulting in recombinant enzymes of molecular weight of 40.4 kDa, 26.2 kDa and 25.8 kDa, respectively. Recombinant AzrS_32 is soluble by 98%, AzrS_28 by 90%, while AzrS_20 is mostly accumulated as inclusion bodies, that have no activity, although pelB leads the target protein to the periplasmic space, where there is a more favorable environment for folding and disulfide bond formation. By contrast, the protein amount of AzrS_28 (i.e. 278 mg/l) is slightly higher than that of AzrS_32 (i.e. 253 mg/l). Therefore, the plasmid pET28a is considered as the ideal vector for production of AzrS
Shewanella xiamenensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
dependent on, best at 0.125 mM
Shewanella xiamenensis
additional information
AzrS shows activity with both NADH and NADPH. The maximum relative activity of AzrS with 0.875 mM NADPH or NADH is 28.1% and 100%, respectively, indicating that AzrS shows a preference for NADH rather than NADPH
Shewanella xiamenensis
NADH
preferred cofactor
Shewanella xiamenensis
NADPH
low activity
Shewanella xiamenensis
General Stability (protein specific)
General Stability
Organism
AzrS is an oxygen-insensitive, FMN-dependent, robust against to organic solvent and has extremely high and stable activity at room temperature
Shewanella xiamenensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
loss of 30.6% activity at 10% v/v
Shewanella xiamenensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten steady-state kinetics
Shewanella xiamenensis
0.1056
-
Methyl orange
recombinant enzyme, pH 6.5, 22C
Shewanella xiamenensis
0.1593
-
Methyl red
recombinant enzyme, pH 6.5, 22C
Shewanella xiamenensis
0.2792
-
Congo red
recombinant enzyme, pH 6.5, 22C
Shewanella xiamenensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates at 5 mM
Shewanella xiamenensis
Co2+
activates at 5 mM
Shewanella xiamenensis
Cr3+
activates at 5 mM
Shewanella xiamenensis
Cu2+
activates at 5 mM
Shewanella xiamenensis
Fe3+
activates at 5 mM
Shewanella xiamenensis
K+
activates at 5 mM
Shewanella xiamenensis
Mg2+
41% activation at 5 mM, best activating cation
Shewanella xiamenensis
Mn2+
activates at 5 mM
Shewanella xiamenensis
Na+
activates at 5 mM
Shewanella xiamenensis
Zn2+
activates at 5 mM
Shewanella xiamenensis
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
1-butanol
loss of 85.4% activity at 10% v/v
Shewanella xiamenensis
1-propanol
loss of 40.9% activity at 10% v/v
Shewanella xiamenensis
Acetone
at 10% v/v, the enzyme is completely stable
Shewanella xiamenensis
chloroform
loss of 55.6% activity at 10% v/v
Shewanella xiamenensis
DMSO
loss of 12.2% activity at 10% v/v
Shewanella xiamenensis
Ethanol
loss of 7.6% activity at 10% v/v
Shewanella xiamenensis
isobutanol
loss of 73.8% activity at 10% v/v
Shewanella xiamenensis
isopropanol
gain of 5.8% activity at 10% v/v
Shewanella xiamenensis
Methanol
at 10% v/v, the enzyme is completely stable
Shewanella xiamenensis
additional information
AzrS is an oxygen-insensitive, FMN-dependent, robust against to organic solvent and has extremely high and stable activity at room temperature
Shewanella xiamenensis
N,N-dimethylformamide
loss of 3.0% activity at 10% v/v
Shewanella xiamenensis
tert-Butanol
loss of 19.0% activity at 10% v/v
Shewanella xiamenensis
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Shewanella xiamenensis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
254
-
purified recombinant enzyme, pH 6.5, 22C, substrate congo red
Shewanella xiamenensis
5152
-
purified recombinant enzyme, pH 6.5, 22C, substrate methl orange
Shewanella xiamenensis
97153
-
purified recombinant enzyme, pH 6.5, 22C, substrate methl red
Shewanella xiamenensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Congo red + NADH + H+
-
743182
Shewanella xiamenensis
? + NAD+
-
-
-
?
Congo red + NADH + H+
-
743182
Shewanella xiamenensis BC01
? + NAD+
-
-
-
?
methyl orange + NADH + H+
-
743182
Shewanella xiamenensis
? + NAD+
-
-
-
?
methyl orange + NADH + H+
-
743182
Shewanella xiamenensis BC01
? + NAD+
-
-
-
?
methyl red + NADH + H+
i.e. 4-dimethylaminoazobenzene-2'-carboxylic acid
743182
Shewanella xiamenensis
anthranilate + N,N'-dimethylaminoaniline + NAD+
-
-
-
?
methyl red + NADH + H+
i.e. 4-dimethylaminoazobenzene-2'-carboxylic acid
743182
Shewanella xiamenensis BC01
anthranilate + N,N'-dimethylaminoaniline + NAD+
-
-
-
?
additional information
substrate specificity: the catalytic efficiency of AzrS follows the descending order of methyl red, methyl orange, and congo red. The enzyme has a wide substrate range of azo dyes
743182
Shewanella xiamenensis
?
-
-
-
-
additional information
substrate specificity: the catalytic efficiency of AzrS follows the descending order of methyl red, methyl orange, and congo red. The enzyme has a wide substrate range of azo dyes
743182
Shewanella xiamenensis BC01
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 21300, about, sequence calculation, x * 40400, recombinant AzrS_32 with TrxA, DS-PAGE, x * 26200, recombinant untagged AzrS_28, SDS-PAGE, x * 25800, recombinant AzrS_20 with pelB signal peptide
Shewanella xiamenensis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
30
-
Shewanella xiamenensis
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
50
activity range, profile overview
Shewanella xiamenensis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
10
50
most stable at 20C, loss of activity at 60C
Shewanella xiamenensis
22
-
AzrS is an oxygen-insensitive, FMN-dependent, robust against to organic solvent and has extremely high and stable activity at room temperature
Shewanella xiamenensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Shewanella xiamenensis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
activity range, profile overview
Shewanella xiamenensis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
most stable at pH 7.0-8.0, loss of activity at pH 5.0 and below
Shewanella xiamenensis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Shewanella xiamenensis
sequence calculation
-
5.23
General Information
General Information
Commentary
Organism
additional information
homologous modeling and molecular docking of AzrS for protein-ligand interactions analysis, using the the azoreductase structure from Escherichia coli strain JM109, PDB ID 1v4b. Methyl red and methyl orange formed the hydrogen bonds with His143, Gly140, Gly141 and Asn176
Shewanella xiamenensis
General Information (protein specific)
General Information
Commentary
Organism
additional information
homologous modeling and molecular docking of AzrS for protein-ligand interactions analysis, using the the azoreductase structure from Escherichia coli strain JM109, PDB ID 1v4b. Methyl red and methyl orange formed the hydrogen bonds with His143, Gly140, Gly141 and Asn176
Shewanella xiamenensis
Other publictions for EC 1.7.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742203
Chalansonnet
Identification of Enterococcu ...
Enterococcus faecalis
BMC Microbiol.
17
126
2017
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
1
1
-
1
-
1
-
-
4
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
1
-
1
-
1
-
-
-
-
2
2
-
-
-
742211
Ryan
-
Azoreductases in drug metabol ...
Bacillus sp. B29, Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Pseudomonas putida, Rhodobacter sphaeroides
Br. J. Pharmacol.
174
2161-2173
2017
-
7
7
3
-
-
7
-
7
-
-
-
-
7
-
-
-
7
-
-
-
-
11
1
-
-
-
-
-
-
-
8
-
-
-
-
7
7
8
3
-
-
-
7
-
-
7
-
-
-
-
-
-
-
-
-
-
-
11
1
-
-
-
-
-
-
-
-
-
15
15
-
-
-
742393
Liu
Exclusive extracellular biore ...
Geobacter sulfurreducens, Geobacter sulfurreducens PCA
Environ. Sci. Technol.
51
8616-8623
2017
-
1
-
-
1
-
-
1
1
-
-
2
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
742660
Rathod
-
Efficient approach to enhance ...
Enterococcus sp., Enterococcus sp. L2
Int. Biodeter. Biodegrad.
124
91-100
2017
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
15
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
741799
Mani
-
Accelerated production of oxy ...
Pseudomonas sp. B1
Asian J. Chem.
28
2562-2570
2016
-
-
-
-
-
-
-
2
1
-
1
-
-
1
1
-
1
-
-
1
1
-
1
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
1
-
1
-
-
1
-
1
-
1
1
-
1
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
742324
Imran
-
Characterization and purifica ...
Shewanella sp. IFN4
CSAWAC
44
1523-1530
2016
4
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
1
-
-
-
2
-
6
-
1
1
3
-
1
1
-
1
-
-
-
4
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
2
-
6
-
1
1
3
-
1
1
-
-
-
2
2
-
-
-
742921
Haghshenas
Molecular dynamics study of b ...
Bacillus sp. B29
J. Biomol. Struct. Dyn.
34
453-462
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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1
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743853
Feng
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Interaction between flavin mo ...
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Spectrosc. Lett.
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2
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1
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1
1
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743182
Zhang
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Cloning and characterization ...
Shewanella xiamenensis, Shewanella xiamenensis BC01
J. Taiwan Inst. Chem. Eng.
61
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2015
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1
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12
2
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1
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8
1
1
1
2
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1
1
1
1
-
1
1
-
-
-
742663
Lu
-
Construction, expression and ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Int. Biodeter. Biodegrad.
87
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2014
2
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1
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1
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7
2
-
2
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2
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1
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1
1
1
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7
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1
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2
-
2
1
1
1
-
2
1
1
2
-
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2
2
743650
Brissos
Improving kinetic or thermody ...
Pseudomonas putida, Pseudomonas putida MET94
PLoS ONE
9
e87209
2014
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1
-
21
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1
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6
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1
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21
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1
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1
1
1
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1
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1
1
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724384
Langer
The flavoenzyme azobenzene red ...
Escherichia coli
Biochemistry
52
4288-4295
2013
-
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4
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3
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1
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724733
Chevalier
The first latent green fluorop ...
Escherichia coli
Chem. Commun. (Camb. )
49
8815-8817
2013
-
1
-
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1
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-
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724864
Yang
Characterization of an efficie ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Environ. Sci. Pollut. Res. Int.
20
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2013
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1
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1
4
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1
1
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6
2
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2
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4
1
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1
1
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2
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1
2
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1
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4
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1
1
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6
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2
-
4
1
-
-
-
-
1
1
-
-
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4
4
726356
Aranganathan
Statistical optimization of sy ...
Pseudomonas oleovorans
Prep. Biochem. Biotechnol.
43
649-667
2013
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1
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1
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1
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2
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1
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1
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1
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1
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1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742097
Yang
Construction of an integrated ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Biores. Technol.
130
517-521
2013
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1
1
-
-
-
-
-
-
-
-
-
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12
-
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1
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-
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4
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1
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1
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1
1
1
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1
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4
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
742662
Shah
-
Community genomics isolation, ...
bacterium enrichment culture clone V9DM
Int. Biodeter. Biodegrad.
79
1-8
2013
-
1
1
-
-
-
-
-
-
-
-
-
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1
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2
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1
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1
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2
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1
1
2
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-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
723925
Morrison
Identification, Isolation and ...
Clostridium perfringens
Anaerobe
18
229-234
2012
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1
-
-
-
1
3
-
-
2
-
-
1
-
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-
-
-
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1
1
1
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1
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2
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1
2
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1
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3
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2
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-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
724120
Feng
Probing the NADH- and Methyl R ...
Enterococcus faecalis
Arch. Biochem. Biophys.
520
99-107
2012
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1
1
13
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-
21
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-
-
-
-
1
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1
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-
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11
-
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2
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1
2
1
13
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-
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21
-
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-
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-
-
-
1
-
-
-
-
11
-
-
-
-
-
-
-
-
21
21
724799
Devi
Homology modeling and function ...
Nostoc sp.
Interdiscip. Sci.
4
310-318
2012
-
-
-
1
-
-
-
-
-
-
-
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1
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3
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3
1
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-
-
-
-
-
-
-
-
-
-
725732
Ooi
-
Flavin-binding of azoreductase ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) B29
J. Mol. Catal. B
74
204-208
2012
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1
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-
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2
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2
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-
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2
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2
1
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2
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1
2
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2
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2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
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725805
Shobana
-
Evolutionary trace analysis of ...
Bacillus subtilis
J. Proteomics Bioinform.
5
222-225
2012
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-
-
1
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-
-
-
-
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1
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1
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-
-
-
-
-
-
-
-
-
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726415
Cui
-
Purification and characterizat ...
Escherichia coli, Escherichia coli CD-2
Process Biochem.
47
544-549
2012
-
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1
-
-
-
5
2
-
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1
-
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2
-
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1
-
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-
-
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6
1
1
1
-
-
1
1
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2
-
-
-
-
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1
2
-
-
-
-
5
-
2
-
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1
-
-
-
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1
-
-
-
-
6
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
724007
Johansson
BTI1, an azoreductase with pH- ...
Bacillus sp. (in: Bacteria) OY1-2, Staphylococcus aureus
Appl. Environ. Microbiol.
77
4223-4225
2011
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-
-
-
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-
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4
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2
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11
-
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5
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4
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-
-
-
11
-
-
-
-
-
5
-
-
-
-
-
-
-
-
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711584
Chen
Identification and molecular c ...
Pigmentiphaga kullae, Pigmentiphaga kullae K24, ATCC BAA-795
BMC Biochem.
11
13
2010
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-
1
-
-
-
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4
-
-
2
-
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2
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1
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-
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2
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8
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
4
-
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2
-
-
-
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1
-
-
2
-
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
724045
Matsumoto
Characterization of thermostab ...
Geobacillus stearothermophilus
Appl. Microbiol. Biotechnol.
86
1431-1438
2010
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1
-
-
-
-
-
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2
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1
-
-
-
-
-
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2
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6
1
1
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2
-
-
-
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4
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
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2
-
-
-
-
-
-
-
2
-
6
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
726423
Ryan
Reaction mechanism of azoreduc ...
Pseudomonas aeruginosa
Protein Cell
1
780-790
2010
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-
-
-
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2
12
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-
-
-
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3
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1
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-
-
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4
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-
9
-
-
-
3
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-
-
-
-
-
3
-
-
-
-
2
-
12
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
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9
-
-
-
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2
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2
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726439
Feng
Evidence for significantly enh ...
Enterococcus faecalis
Protein Pept. Lett.
17
578-584
2010
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1
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1
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3
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-
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-
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1
1
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-
-
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1
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-
-
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-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
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696845
Ooi
Comparative enzymatic analysis ...
Bacillus sp. B29
Biosci. Biotechnol. Biochem.
73
1209-1211
2009
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1
1
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-
-
-
4
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2
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2
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1
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-
-
3
-
16
1
2
-
-
-
1
2
-
2
-
-
-
-
2
2
4
-
-
-
-
-
-
4
-
-
2
-
-
-
-
2
-
-
4
-
16
2
2
-
-
-
2
2
-
-
-
-
-
-
-
-
697494
Punj
Purification and identificatio ...
Enterococcus faecalis
Curr. Issues Mol. Biol.
11
59-65
2009
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-
-
-
-
-
-
4
-
-
-
-
-
2
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-
1
-
-
-
4
-
1
-
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
4
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
697495
Macwana
Identification and Isolation o ...
Enterococcus faecium, Enterococcus faecium ATCC 6569
Curr. Issues Mol. Biol.
12
43-48
2009
-
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1
-
-
-
-
3
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1
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-
6
-
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1
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-
-
18
-
19
1
2
-
-
2
2
-
-
3
-
-
-
-
-
2
5
-
-
-
-
-
-
4
-
-
2
-
-
-
-
1
-
-
20
-
19
1
2
-
-
4
2
-
-
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-
-
-
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2
2
698629
Liu
The Escherichia coli azoreduct ...
Escherichia coli
J. Bacteriol.
191
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2009
5
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1
-
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-
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4
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1
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1
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-
-
-
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4
1
-
-
-
4
1
-
-
2
-
-
-
5
-
1
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
4
1
-
-
-
4
1
-
-
-
-
-
-
-
-
-
710749
Wang
Role of tyrosine 131 in the ac ...
Pseudomonas aeruginosa
Acta Crystallogr. Sect. F
66
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2009
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1
1
1
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8
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2
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1
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-
-
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7
-
-
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1
8
-
-
-
2
-
-
-
-
-
1
2
1
1
-
-
-
-
8
-
-
-
-
-
-
-
1
-
-
-
-
7
-
-
-
1
8
-
-
-
-
-
-
-
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711798
Punj
Purification and identificatio ...
Enterococcus faecalis, Enterococcus faecalis ATCC 27274
Curr. Issues Mol. Biol.
11
59-66
2009
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-
-
-
-
-
3
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4
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-
1
-
-
-
2
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
695789
Bafana
Azoreductase and dye detoxific ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens AB
Appl. Microbiol. Biotechnol.
77
1139-1144
2008
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1
-
-
-
-
-
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1
-
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2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
695803
Liu
Enhancing survival of Escheric ...
Rhodobacter sphaeroides
Appl. Microbiol. Biotechnol.
80
409-416
2008
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1
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-
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1
4
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-
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1
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1
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-
-
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4
-
1
-
-
4
2
1
-
1
1
-
-
-
-
1
1
-
-
-
-
1
1
4
-
-
-
-
-
-
-
1
-
-
-
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4
-
1
-
-
4
2
1
-
-
-
-
-
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-
696793
Sandhya
Decolorizing kinetics of a rec ...
Brevibacillus laterosporus, Brevibacillus laterosporus RRK1
Biores. Technol.
99
2187-2191
2008
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1
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1
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10
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1
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696902
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Site-directed mutagenesis of s ...
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1
1
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3
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697473
Bafana
Lateral gene transfer in phylo ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) OY1-2, Bacillus subtilis 168, Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Geobacillus stearothermophilus, Pigmentiphaga kullae, Pigmentiphaga kullae K24, Rhodobacter sphaeroides, Rhodobacter sphaeroides AS1.1737, Staphylococcus aureus, Xenophilus azovorans, Xenophilus azovorans KF46F / DSM 13620
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1
1
3
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4
1
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700040
Chen
Functional role of Trp-105 of ...
Enterococcus faecalis
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2008
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1
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6
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10
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1
1
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1
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671554
Xu
Fe(III)-enhanced Azo Reduction ...
Shewanella decolorationis, Shewanella decolorationis S12
Appl. Microbiol. Biotechnol.
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1342-1349
2007
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4
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673224
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Recent advances in azo dye deg ...
Acidaminococcus fermentans, Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) OY-2, Bacillus sp. (in: Bacteria) SF, Bacillus subtilis, Bacteroides thetaiotaomicron, Bifidobacterium adolescentis, Blautia obeum, Citrobacter sp., Clostridium clostridioforme, Clostridium perfringens, Coprococcus catus, Enterococcus faecalis, Escherichia coli, Fusobacterium sp., Holdemanella biformis, Kocuria rosea, Kocuria varians, Micrococcus luteus, Pantoea agglomerans, Pigmentiphaga kullae, Pigmentiphaga kullae K24, Proteus vulgaris, Rhodobacter sphaeroides, Shigella dysenteriae, Staphylococcus aureus, Staphylococcus epidermidis, Xenophilus azovorans, Xenophilus azovorans KF46
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64
9
10
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17
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17
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17
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64
9
10
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10
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674607
Ito
Three-dimensional structure of ...
Escherichia coli
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2006
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1
1
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658574
Nachiyar
-
Purification and characterizat ...
Pseudomonas aeruginosa
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1
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3
2
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1
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1
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7
1
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7
1
1
1
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1
1
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671417
Ramalho
Azo reductase activity of inta ...
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2005
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Biochemical and molecular char ...
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2
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2
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6
1
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2
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1
2
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1
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2
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6
1
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657603
Maier
A new alkali-thermostable azor ...
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12
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1
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3
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1
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2
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1
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1
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2
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1
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1
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1
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-
-
12
-
1
-
3
-
1
-
-
1
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658773
Yan
Expression and characteristics ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides AS1.1737
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236
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2004
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1
1
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2
4
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2
2
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6
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12
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2
1
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1
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1
1
1
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2
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4
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2
2
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1
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6
-
12
-
2
1
-
-
-
-
-
-
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660430
Chen
Molecular cloning, overexpress ...
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1
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1
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7
1
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2
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1
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1
2
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1
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1
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1
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7
1
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-
-
-
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-
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1
-
-
-
-
-
-
657620
Bluemel
Cloning and characterization o ...
Pigmentiphaga kullae, Pigmentiphaga kullae K24
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2003
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1
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2
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5
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1
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1
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6
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2
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1
1
2
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2
2
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1
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1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
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Moutaouakkil
Purification and partial chara ...
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139-146
2003
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1
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-
-
-
5
2
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1
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1
-
-
1
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-
-
-
1
5
1
1
1
-
-
1
1
-
1
-
-
5
-
1
-
1
-
-
-
5
5
-
2
-
-
1
-
-
-
-
1
-
-
-
1
5
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
657580
Blumel
Molecular cloning and characte ...
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Appl. Environ. Microbiol.
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2002
-
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1
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-
-
-
-
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3
2
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6
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1
-
-
-
1
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4
1
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-
-
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3
-
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1
1
3
-
-
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-
-
-
-
-
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3
2
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-
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
394314
Suzuki
Molecular cloning and characte ...
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J. Biol. Chem.
276
9059-9065
2001
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-
1
-
-
-
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1
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1
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1
-
-
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2
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3
1
2
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-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
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-
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1
-
-
-
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1
-
-
2
-
3
1
2
-
-
-
-
-
-
-
-
-
-
-
-
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394313
Mazumder
-
Characteristics and purificati ...
Brevundimonas subvibrioides
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476-483
1999
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1
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1
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1
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2
1
-
1
1
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-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
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6
-
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1
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-
-
1
-
2
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
394312
Singh
Azo reductase activity of micr ...
Canis lupus familiaris, Capra hircus, Cavia porcellus, Cricetinae, Felis catus, Gallus gallus, Macaca mulatta, Mus musculus, Oryctolagus cuniculus, Ovis aries, Pigeon, Rattus norvegicus
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33
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33
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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394311
Singh
Comparative azo reductase acti ...
Rattus norvegicus
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35
1016-1018
1997
-
-
-
-
-
-
-
-
-
-
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3
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-
1
6
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-
-
-
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-
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-
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-
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-
1
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
394310
Ghosh
Purification of two azoreducta ...
Escherichia coli
Indian J. Exp. Biol.
31
951-954
1993
1
-
-
-
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2
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3
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1
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8
-
4
1
1
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-
-
1
-
-
4
-
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-
1
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-
4
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-
-
-
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2
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3
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1
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8
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
394300
Rafii
Azoreductase activity of anaer ...
Anaerostipes hadrus, Bacteroides sp., Butyrivibrio sp., Clostridium clostridioforme, Clostridium paraputrificum, Clostridium perfringens, Clostridium sp., Eubacterium sp., Tyzzerella nexilis
Appl. Environ. Microbiol.
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1990
-
-
-
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-
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9
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9
9
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17
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9
-
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9
-
-
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9
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9
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17
-
-
-
-
-
-
-
-
-
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-
-
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394299
Zimmermann
Comparison of two bacterial az ...
Pseudomonas sp., Pseudomonas sp. K24
Arch. Microbiol.
138
37-43
1984
-
-
-
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1
6
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2
2
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3
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1
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2
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10
1
1
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2
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2
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2
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-
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1
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6
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2
2
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1
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2
-
10
1
1
-
-
-
2
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394301
Moreno
Ca2+ and Mg2+-enhanced reducti ...
Rattus norvegicus
J. Biol. Chem.
259
14609-14616
1984
-
-
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2
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1
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2
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1
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2
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394298
Zimmermann
Properties of purified Orange ...
Pseudomonas sp., Pseudomonas sp. KF46
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129
197-203
1982
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1
18
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2
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2
1
21
1
1
1
1
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2
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2
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1
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18
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2
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1
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2
1
21
1
1
1
1
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2
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394302
Fujita
The stimulation of microsomal ...
Rattus norvegicus
Biochim. Biophys. Acta
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178-189
1982
2
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1
1
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1
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3
1
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2
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3
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1
1
1
1
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1
-
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2
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Mallett
A continuous spectrophotometri ...
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1
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1
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2
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2
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2
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1
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2
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1
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2
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2
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Rat liver cytosolic azoreducta ...
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254
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1
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1
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1
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2
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1
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1
1
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2
1
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1
1
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Huang
Rat liver cytosolic azoreducta ...
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7
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1
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7
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Mallett
The apparent non-identity of c ...
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2
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1
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Fujita
Liver microsomal cytochromes P ...
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394295
Matsudomi
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1
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4
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1
1
2
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1
1
5
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1
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5
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7
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1
1
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1
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1
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4
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1
1
2
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1
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Mallett
A continuous assay for hepatic ...
Rattus norvegicus
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1
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1
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394307
Smith
1,2-Dimethyl-4-(p-carboxypheny ...
Canis lupus familiaris, Cavia porcellus, Gallus gallus, Microtus pennsylvanicus, Rattus norvegicus
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5
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5
5
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10
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394308
Daniel
A soluble aerobic reduced nico ...
Rattus norvegicus
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1969
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1
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1
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394309
Ketterer
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The effect of 4-dimethylaminoa ...
Rattus norvegicus
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1968
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