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Literature summary for 1.7.1.6 extracted from

  • Nakhaee, N.; Asad, S.; Khajeh, K.; Arab, S.; Amoozegar, M.
    Improving the thermal stability of azoreductase from Halomonas elongata by introducing a disulfide bond via site-directed mutagenesis (2018), Biotechnol. Appl. Biochem., 65, 883-891 .
    View publication on PubMed
Search for more literature for 1.7.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) and Rosetta-gami (DE3) cells Halomonas elongata

Protein Variants

Protein Variants Comment Organism
L49C/D108C the mutant enzyme shows increased thermal stability at 50°C (increased half-life from 12.6 min in wild type to 26.66 min in the mutant enzyme). The mutant enzyme can also tolerate 5% (w/v) NaCl and retains 30% of original activity after 24 h incubation Halomonas elongata

Inhibitors

Inhibitors Comment Organism Structure
NaCl the wild type enzyme is completely inactivated by 5% (w/v) NaCl after 24 h incubation Halomonas elongata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 Methyl red wild type enzyme, at pH 7.6 and 25°C Halomonas elongata
0.044
-
 Methyl red mutant enzyme L49C/D108C, at pH 7.6 and 25°C Halomonas elongata
0.091
-
 NADH wild type enzyme, at pH 7.6 and 25°C Halomonas elongata
0.223
-
 NADH mutant enzyme L49C/D108C, at pH 7.6 and 25°C Halomonas elongata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration Halomonas elongata

Organism

Organism UniProt Comment Textmining
Halomonas elongata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Halomonas elongata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl red + NADH
-
 Halomonas elongata 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 22000, SDS-PAGE Halomonas elongata

Synonyms

Synonyms Comment Organism
AzoH
-
 Halomonas elongata
azoreductase
-
 Halomonas elongata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life of 12.6 min at 50°C Halomonas elongata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.048
-
 NADH mutant enzyme L49C/D108C, at pH 7.6 and 25°C Halomonas elongata
0.048
-
 Methyl red mutant enzyme L49C/D108C, at pH 7.6 and 25°C Halomonas elongata
0.148
-
 NADH wild type enzyme, at pH 7.6 and 25°C Halomonas elongata
0.148
-
 Methyl red wild type enzyme, at pH 7.6 and 25°C Halomonas elongata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Halomonas elongata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.215
-
 NADH mutant enzyme L49C/D108C, at pH 7.6 and 25°C Halomonas elongata
1.01
-
 Methyl red mutant enzyme L49C/D108C, at pH 7.6 and 25°C Halomonas elongata
1.62
-
 NADH wild type enzyme, at pH 7.6 and 25°C Halomonas elongata
11.38
-
 Methyl red wild type enzyme, at pH 7.6 and 25°C Halomonas elongata