Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Bacillus velezensis | |
dithiothreitol | - |
Bacillus velezensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus velezensis |
Crystallization (Comment) | Organism |
---|---|
using 50 mM monobasic potassium phosphate and 20% polyethylene glycol 8000 | Bacillus velezensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is oxygen-insensitive | Bacillus velezensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69900 | - |
SEC-MALS | Bacillus velezensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus velezensis | B3VPZ9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Bacillus velezensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Congo Red + NADPH + H+ | the enzyme decolorizes 9% of 0.04 mM Congo Red in 15 min | Bacillus velezensis | ? | - |
? | |
Methyl Orange + NADPH + H+ | the enzyme decolorizes more than 14% of 0.04 mM Methyl Orange in 15 min | Bacillus velezensis | ? | - |
? | |
methyl red + NADH + H+ | the rate of reaction is about 7.14 times faster with NADPH at different concentrations of Methyl Orange as compared to that with NADH | Bacillus velezensis | ? | - |
? | |
methyl red + NADPH + H+ | the enzyme decolorizes more than 50% of 0.04 mM Methyl Red in 15 min | Bacillus velezensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 20000, SDS-PAGE | Bacillus velezensis |
Synonyms | Comment | Organism |
---|---|---|
azoreductase | - |
Bacillus velezensis |
YhdA | - |
Bacillus velezensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus velezensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | at high temperature of 50°C, the enzyme shows about 79% activity of that at 37°C. On further increasing the temperature to 60°C, the activity drops to 64%, while the activity is 80% at low temperature of 20°C | Bacillus velezensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
melting temperature of 89.5°C | Bacillus velezensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Bacillus velezensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Bacillus velezensis | |
NADPH | favored electron donor | Bacillus velezensis |