BRENDA - Enzyme Database
show all sequences of 1.7.1.B1

OYE flavoprotein reductases initiate the condensation of TNT-derived intermediates to secondary diarylamines and nitrite

Wittich, R.M.; Haidour, A.; Van Dillewijn, P.; Ramos, J.L.; Environ. Sci. Technol. 42, 734-739 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
For His-tagged protein production, plasmid is transformed into Escherichia coli BL21 pLysS.
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NH4Cl
included in assay medium
Pseudomonas putida
Zn2+
included in assay medium
Pseudomonas putida
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida JLR11
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by nickel affinity chromatography using a continuous imidazole gradient
Pseudomonas putida
Reaction
Reaction
Commentary
Organism
Reaction ID
2,4,6-trinitrotoluene + 2 NADPH + 2 H+ = N-hydroxy-2-methyl-3,5-dinitroaniline + 2 NADP+ + H2O
The amount of N-hydroxy-2-methyl-3,5-dinitroaniline is larger than the amount of N-hydroxy-4-methyl-3,5-dinitroaniline formed. In a further nonenzymatic reaction nitrite is released and N-(2-methyl-3,5-dinitrophenyl)-N-4-methyl-3,5-dinitrophenyl-hydroxylamine and eventually N-(2-methyl-3,5-dinitrophenyl)-N-4-methyl-3,5-dinitroaniline are produced. In adidtion the aromatic ring of 2,4,6-trinitrotoluene is susceptible to nucleophilic attack by hydride ions to form Meisenheimer complex intermediates (monohydride and dihydride complexes) which also form the secondary diaryl hydroxylamines and the secondary diarylamine with release of nitrite
Pseudomonas putida
2,4,6-trinitrotoluene + 2 NADPH + 2 H+ = N-hydroxy-4-methyl-3,5-dinitroaniline + 2 NADP+ + H2O
The amount of N-hydroxy-4-methyl-3,5-dinitroaniline is larger than the amount of N-hydroxy-2-methyl-3,5-dinitroaniline formed. In a further nonenzymatic reaction nitrite is released and N,N-bis-(3,5-dinitrotolyl)-hydroxylamine and eventually N,N-bis-(3,5-dinitrotolyl)-amine are produced. In addition the aromatic ring of 2,4,6-trinitrotoluene is susceptible to nucleophilic attack by hydride ions to form Meisenheimer complex intermediates (monohydride and dihydride complexes) which also form the secondary diaryl hydroxylamines and the secondary diarylamine with release of nitrite
Pseudomonas putida
Synonyms
Synonyms
Commentary
Organism
flavoprotein reductase
-
Pseudomonas putida
xenobiotic reductase B
-
Pseudomonas putida
xenobiotic reductase B (XenB)-like flavin reductase
-
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
For His-tagged protein production, plasmid is transformed into Escherichia coli BL21 pLysS.
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NH4Cl
included in assay medium
Pseudomonas putida
Zn2+
included in assay medium
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
by nickel affinity chromatography using a continuous imidazole gradient
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pseudomonas putida
Other publictions for EC 1.7.1.B1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723954
Fetar
mexEF-oprN multidrug efflux op ...
Pseudomonas aeruginosa
Antimicrob. Agents Chemother.
55
508-514
2011
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1
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2
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2
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690550
Van Dillewijn
Subfunctionality of hydride tr ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
74
6703-6708
2008
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1
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5
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1
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3
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1
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2
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11
1
4
1
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2
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1
2
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1
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11
1
1
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1
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690551
Van Dillewijn
Type II hydride transferases f ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
74
6820-6823
2008
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2
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1
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692112
Wittich
OYE flavoprotein reductases in ...
Pseudomonas putida, Pseudomonas putida JLR11
Environ. Sci. Technol.
42
734-739
2008
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1
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2
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4
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1
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1
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1
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1
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740929
Ho
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Characterization of nitroreduc ...
Pseudomonas sp.
Korean J. Microbiol. Biotechnol.
32
230-237
2004
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5
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1
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1
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1
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1
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1
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1
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5
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1
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1
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1
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