BRENDA - Enzyme Database
show all sequences of 1.7.1.B1

Characterization of nitroreductase purified from TNT-degrading bacterium, Pseudomonas sp. HK-6

Ho, E.; Kahng, H.; Oh, K.; Korean J. Microbiol. Biotechnol. 32, 230-237 (2004)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Pseudomonas sp.
Ag+
about 70% inhibition
Pseudomonas sp.
Cu2+
about 70% inhibition
Pseudomonas sp.
Hg2+
about 70% inhibition
Pseudomonas sp.
additional information
not inhibitory: Fe2+
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
x * 27000, SDS-PAGE
Pseudomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.01
-
pH 7.0, 30°C
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,4,6-trinitrotoluene + 2 NADPH + 2 H+
-
740929
Pseudomonas sp.
N-hydroxy-2-methyl-3,5-dinitroaniline + 2 NADP+ + H2O
-
-
-
?
additional information
enzyme additionally catalyzes reduction of nitrobenzene, reaction of EC 1.7.1.16
740929
Pseudomonas sp.
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 27000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Pseudomonas sp.
Ag+
about 70% inhibition
Pseudomonas sp.
Cu2+
about 70% inhibition
Pseudomonas sp.
Hg2+
about 70% inhibition
Pseudomonas sp.
additional information
not inhibitory: Fe2+
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
x * 27000, SDS-PAGE
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.01
-
pH 7.0, 30°C
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,4,6-trinitrotoluene + 2 NADPH + 2 H+
-
740929
Pseudomonas sp.
N-hydroxy-2-methyl-3,5-dinitroaniline + 2 NADP+ + H2O
-
-
-
?
additional information
enzyme additionally catalyzes reduction of nitrobenzene, reaction of EC 1.7.1.16
740929
Pseudomonas sp.
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 27000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas sp.
Other publictions for EC 1.7.1.B1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723954
Fetar
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508-514
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2
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2
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690550
Van Dillewijn
Subfunctionality of hydride tr ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
74
6703-6708
2008
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-
1
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5
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11
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11
1
1
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1
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690551
Van Dillewijn
Type II hydride transferases f ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
74
6820-6823
2008
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2
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4
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1
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1
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692112
Wittich
OYE flavoprotein reductases in ...
Pseudomonas putida, Pseudomonas putida JLR11
Environ. Sci. Technol.
42
734-739
2008
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-
1
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2
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3
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1
2
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3
1
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1
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1
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2
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1
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1
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1
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740929
Ho
-
Characterization of nitroreduc ...
Pseudomonas sp.
Korean J. Microbiol. Biotechnol.
32
230-237
2004
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5
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1
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1
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1
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5
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1
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