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Literature summary for 1.7.1.B1 extracted from
- Evaluation of two xenobiotic reductases from Pseudomonas putida for their suitability for magnetic nanoparticle-directed enzyme prodrug therapy as a novel approach to cancer treatment (2020), MicrobiologyOpen, 9, e1110 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta pLysS cells | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.457 | - |
CB1954 | genetically modified protein XenB-cys, at pH 7.2 temperature not specified in the publication | Pseudomonas putida | |
| 1.69 | - |
CB1954 | genetically modified protein XenB-his, at pH 7.2 temperature not specified in the publication | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CB1954 + NADPH + H+ | - |
Pseudomonas putida | ? + hydroxylamine + NADP+ | - |
? |  |
| additional information | the enzyme shows no reactivity with CB1954 | Pseudomonas putida | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 39000, SDS-PAGE | Pseudomonas putida |
| homodimer | 2 * 40000, SDS-PAGE | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XenA | - |
Pseudomonas putida |
| XenB | - |
Pseudomonas putida |
| xenobiotic reductase A | - |
Pseudomonas putida |
| xenobiotic reductase B | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
CB1954 | genetically modified protein XenB-cys, at pH 7.2 temperature not specified in the publication | Pseudomonas putida | |
| 8.2 | - |
CB1954 | genetically modified protein XenB-his, at pH 7.2 temperature not specified in the publication | Pseudomonas putida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.9 | - |
CB1954 | genetically modified protein XenB-cys, at pH 7.2 temperature not specified in the publication | Pseudomonas putida | |
| 4.9 | - |
CB1954 | genetically modified protein XenB-his, at pH 7.2 temperature not specified in the publication | Pseudomonas putida |
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