F123A
site-directed mutagenesis, residue F123 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme
Escherichia coli
F124A
site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme
Escherichia coli
F124N
site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme
Escherichia coli
F124W
site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme
Escherichia coli
N71S
site-directed mutagenesis, active site residue mutation, the mutant shows increased activity with prodrug 5-[aziridin-1-yl]-2,4-dinitrobenzamide (CB1954) and nitrofurazone compared to the wild-type enzyme
Escherichia coli
T41L
site-directed mutagenesis, active site residue mutation, the mutant shows increased activity with prodrug 5-[aziridin-1-yl]-2,4-dinitrobenzamide (CB1954) and nitrofurazone compared to the wild-type enzyme
Escherichia coli
additional information
additional information
Michaelis-Menten steady-state kinetics of recombinant His6-tagged wild-type and mutant enzymes
Escherichia coli
0.0023
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
0.0057
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
0.0072
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
0.0091
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
0.0174
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
0.0398
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
0.0534
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
0.0881
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
0.0928
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
0.0996
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
0.0997
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
0.163
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
0.172
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
0.265
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
0.306
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
0.339
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
0.38
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
0.411
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
0.518
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
0.575
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
0.732
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
1.069
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
1.168
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
1.495
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
2,4,6-trinitrotoluene + NADH + H+
743709
Escherichia coli
4-hydroxyamino-2,6-dinitrotoluene + NAD+ + H2O
?
2,4,6-trinitrotoluene + NADPH + H+
743709
Escherichia coli
4-hydroxyamino-2,6-dinitrotoluene + NADP+ + H2O
?
2,4,6-trinitrotoluene + NADPH + H+
i.e. TNT, NfsB reduces TNT to the para isomer 4-hydroxyamino-2,6-dinitrotoluene more easily than the ortho isomer 2-hydroxyamino-4,6-dinitrotoluene
743709
Escherichia coli
4-hydroxyamino-2,6-dinitrotoluene + NADP+ + H2O
?
2-amino-4,6-dinitrotoluene + NADH + H+
743709
Escherichia coli
4-hydroxyamino-2-aminotoluene + NAD+ + H2O
?
4-amino-2,6-dinitrotoluene + NADH + H+
743709
Escherichia coli
2-hydroxyamino-4-aminotoluene + NAD+ + H2O
?
additional information
no activity with 2,4-diamino-6-nitrotoluene and 2,6-diamino-4-nitrotoluene
743709
Escherichia coli
?
?
0.76
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
1.03
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
1.51
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
3.25
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
4.74
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
8.06
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
9.28
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
9.4
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
11.29
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
11.48
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
14.01
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
14.38
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
15.27
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
15.31
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
18.02
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
18.2
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
26.84
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
26.9
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
37.65
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
38.88
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
46.67
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
54.52
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
62.87
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
69.26
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
F123A
site-directed mutagenesis, residue F123 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme
Escherichia coli
F124A
site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme
Escherichia coli
F124N
site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme
Escherichia coli
F124W
site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme
Escherichia coli
N71S
site-directed mutagenesis, active site residue mutation, the mutant shows increased activity with prodrug 5-[aziridin-1-yl]-2,4-dinitrobenzamide (CB1954) and nitrofurazone compared to the wild-type enzyme
Escherichia coli
T41L
site-directed mutagenesis, active site residue mutation, the mutant shows increased activity with prodrug 5-[aziridin-1-yl]-2,4-dinitrobenzamide (CB1954) and nitrofurazone compared to the wild-type enzyme
Escherichia coli
additional information
additional information
Michaelis-Menten steady-state kinetics of recombinant His6-tagged wild-type and mutant enzymes
Escherichia coli
0.0023
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
0.0057
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
0.0072
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
0.0091
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
0.0174
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
0.0398
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
0.0534
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
0.0881
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
0.0928
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
0.0996
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
0.0997
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
0.163
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
0.172
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
0.265
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
0.306
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
0.339
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
0.38
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
0.411
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
0.518
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
0.575
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
0.732
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
1.069
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
1.168
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
1.495
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
2,4,6-trinitrotoluene + NADH + H+
743709
Escherichia coli
4-hydroxyamino-2,6-dinitrotoluene + NAD+ + H2O
?
2,4,6-trinitrotoluene + NADPH + H+
743709
Escherichia coli
4-hydroxyamino-2,6-dinitrotoluene + NADP+ + H2O
?
2,4,6-trinitrotoluene + NADPH + H+
i.e. TNT, NfsB reduces TNT to the para isomer 4-hydroxyamino-2,6-dinitrotoluene more easily than the ortho isomer 2-hydroxyamino-4,6-dinitrotoluene
743709
Escherichia coli
4-hydroxyamino-2,6-dinitrotoluene + NADP+ + H2O
?
2-amino-4,6-dinitrotoluene + NADH + H+
743709
Escherichia coli
4-hydroxyamino-2-aminotoluene + NAD+ + H2O
?
4-amino-2,6-dinitrotoluene + NADH + H+
743709
Escherichia coli
2-hydroxyamino-4-aminotoluene + NAD+ + H2O
?
additional information
no activity with 2,4-diamino-6-nitrotoluene and 2,6-diamino-4-nitrotoluene
743709
Escherichia coli
?
?
0.76
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
1.03
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
1.51
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
3.25
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
4.74
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
8.06
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
9.28
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
9.4
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
11.29
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
11.48
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
14.01
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
14.38
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
15.27
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
15.31
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
18.02
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
18.2
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
26.84
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
26.9
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
37.65
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
38.88
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
46.67
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
54.52
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
62.87
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
69.26
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
4
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
6
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
6
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
11
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
12
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
26
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
27
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
30
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
31
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
31
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
45
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
46
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
57
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
65
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
181
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
206
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
290
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
547
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
653
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
1177
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
2170
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
2526
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
5111
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
5389
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
4
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
6
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
6
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
11
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
12
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
26
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
27
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
30
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
31
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
31
4-amino-2,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
45
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
46
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
57
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
65
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
181
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli
206
2-amino-4,6-dinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
290
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
Escherichia coli
547
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
Escherichia coli
653
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
Escherichia coli
1177
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
Escherichia coli
2170
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
Escherichia coli
2526
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
Escherichia coli
5111
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
Escherichia coli
5389
2,4,6-trinitrotoluene
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
Escherichia coli