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Literature summary for 1.7.2.1 extracted from

  • Kataoka, K.; Yamaguchi, K.; Kobayashi, M.; Mori, T.; Bokui, N.; Suzuki, S.
    Structure-based engineering of Alcaligenes xylosoxidans copper-containing nitrite reductase enhances intermolecular electron transfer reaction with pseudoazurin (2004), J. Biol. Chem., 279, 53374-53378.
    View publication on PubMed
Search for more literature for 1.7.2.1

Protein Variants

Protein Variants Comment Organism
A191E slight increase in electron transfer rate constant Achromobacter xylosoxidans
A191E/G198E 3fold increase in electron transfer rate constant Achromobacter xylosoxidans
A83D slight increase in electron transfer rate constant Achromobacter xylosoxidans
A83D/A191E 3fold increase in electron transfer rate constant Achromobacter xylosoxidans
A83D/A191E/G198E 4.7fold increase in electron transfer rate constant Achromobacter xylosoxidans
A83D/G198E 3fold increase in electron transfer rate constant Achromobacter xylosoxidans
G198E 2.6fold increase in electron transfer rate constant Achromobacter xylosoxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00008
-
 nitrite 25°C, A83D/A191E/G198E triple mutant Achromobacter xylosoxidans
0.00024
-
 nitrite 25°C, A191E/G198E double mutant Achromobacter xylosoxidans
0.00025
-
 nitrite 25°C, A83D/G198E double mutant Achromobacter xylosoxidans
0.0003
-
 nitrite 25°C, A83D/A191E double mutant Achromobacter xylosoxidans
0.0005
-
 nitrite 25°C, G198E mutant Achromobacter xylosoxidans
0.00096
-
 nitrite 25°C, A191E mutant Achromobacter xylosoxidans
0.001
-
 nitrite 25°C, A83D mutant Achromobacter xylosoxidans
0.0013
-
 nitrite 25°C, wild-type Achromobacter xylosoxidans

Metals/Ions

Metals/Ions Comment Organism Structure
copper copper-containing nitrite reductase Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrite + reduced pseudoazurin Achromobacter xylosoxidans
-
 NO + oxidized pseudoazurin
-
 ?

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + reduced pseudoazurin
-
 Achromobacter xylosoxidans NO + oxidized pseudoazurin
-
 ?
nitrite + reduced pseudoazurin pseudoazurin from Achromobacter cycloclastes Achromobacter xylosoxidans NO + oxidized pseudoazurin
-
 ?

Synonyms

Synonyms Comment Organism
AxNiR
-
 Achromobacter xylosoxidans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
172
-
 nitrite 25°C Achromobacter xylosoxidans