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Literature summary for 1.7.2.1 extracted from

  • Kim, S.W.; Fushinobu, S.; Zhou, S.; Wakagi, T.; Shoun, H.
    Eukaryotic nirK genes encoding copper-containing nitrite reductase: originating from the protomitochondrion? (2009), Appl. Environ. Microbiol., 75, 2652-2658.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.2.1

Inhibitors

Inhibitors Comment Organism Structure
CO 20% residual activity Fusarium oxysporum
diethyldithiocarbamate 1 mM, 10% residual activity Fusarium oxysporum
EDTA 1 mM, 60% resiudal activity Fusarium oxysporum
KCN 1 mM, 5% residual activity Fusarium oxysporum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the first 65 amino acid residues of theN-terminal domain constitute a mitochondrial targeting signal, sequence analysis Fusarium oxysporum 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper ligands of the type 1 and 2 Cu centers are completely conserved in NiR. They are His253, Cys294, His302, and Met307 for the type 1 Cu center and His258, His293, and His456 for type 2.Asp256 and His405 residues are important for enzyme catalysis by forming a hydrogen bond network on the type 2 Cu center. The EPR spectrum is also characteristic of NirK proteins that contain both type 1 and type 2 copper centers Fusarium oxysporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53705
-
 x * 53705, calculated, x * 60000, SDS-PAGE Fusarium oxysporum
60000
-
 x * 53705, calculated, x * 60000, SDS-PAGE Fusarium oxysporum
900000
-
 gel filtration, aggregate Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum A9XR52
-
-

Purification (Commentary)

Purification (Comment) Organism
purified NiR exhibits a blue color spectrum showing maximum absorbance at 594 nm Fusarium oxysporum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
303
-
 pH 6.5, 35°C Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + methyl viologen
-
 Fusarium oxysporum NO + oxidized methyl viologen + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 53705, calculated, x * 60000, SDS-PAGE Fusarium oxysporum

Synonyms

Synonyms Comment Organism
NirK
-
 Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 optimum below pH 6.5 Fusarium oxysporum