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Literature summary for 1.7.2.1 extracted from

  • Kobayashi, S.; Hira, D.; Yoshida, K.; Toyofuku, M.; Shida, Y.; Ogasawara, W.; Yamaguchi, T.; Araki, N.; Oshiki, M.
    Nitric oxide production from nitrite reduction and hydroxylamine oxidation by copper-containing dissimilatory nitrite reductase (NirK) from the aerobic ammonia-oxidizing archaeon, Nitrososphaera viennensis (2018), Microbes Environ., 33, 428-434 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Nitrososphaera viennensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.287
-
 nitrite pH 6.5, 25°C Nitrososphaera viennensis
0.287
-
 nitrite at pH 8.0 and 25°C Nitrososphaera viennensis

Metals/Ions

Metals/Ions Comment Organism Structure
copper 2.9 atoms per subunit Nitrososphaera viennensis
Cu2+ contains 2.9 atoms per subunit of the recombinant protein Nitrososphaera viennensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 gel filtration Nitrososphaera viennensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrite + ferrocytochrome c + 2 H+ Nitrososphaera viennensis
-
 nitric oxide + H2O + ferricytochrome c
-
 ?
nitrite + ferrocytochrome c + 2 H+ Nitrososphaera viennensis JCM19564
-
 nitric oxide + H2O + ferricytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Nitrososphaera viennensis
-
-
-
Nitrososphaera viennensis A0A060HKX3
-
-
Nitrososphaera viennensis EN76 A0A060HKX3
-
-
Nitrososphaera viennensis JCM19564
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His60 Ni Superflow resin column chromatography and Superdex 200 gel filtration Nitrososphaera viennensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes the reduction of NO2- to NO, the oxidation of hydroxylamine (NH2OH) to NO, reaction of EC 1.7.2.6, and the production of N2O from NH2OH and NO2 Nitrososphaera viennensis ?
-
-
additional information enzyme catalyzes the reduction of NO2- to NO, the oxidation of hydroxylamine (NH2OH) to NO, reaction of EC 1.7.2.6, and the production of N2O from NH2OH and NO2 Nitrososphaera viennensis EN76 ?
-
-
nitrite + ferrocytochrome c + 2 H+
-
 Nitrososphaera viennensis nitric oxide + H2O + ferricytochrome c
-
 ?
nitrite + ferrocytochrome c + 2 H+
-
 Nitrososphaera viennensis JCM19564 nitric oxide + H2O + ferricytochrome c
-
 ?
nitrite + reduced benzyl viologen
-
 Nitrososphaera viennensis NO + oxidized benzyl viologen
-
 ?
nitrite + reduced benzyl viologen
-
 Nitrososphaera viennensis EN76 NO + oxidized benzyl viologen
-
 ?
nitrite + reduced benzyl viologen + 2 H+
-
 Nitrososphaera viennensis nitric oxide + H2O + oxidized benzyl viologen
-
 ?
nitrite + reduced benzyl viologen + 2 H+
-
 Nitrososphaera viennensis JCM19564 nitric oxide + H2O + oxidized benzyl viologen
-
 ?
nitrite + reduced phenazine methosulfate
-
 Nitrososphaera viennensis NO + oxidized phenazine methosulfate
-
 ?
nitrite + reduced phenazine methosulfate
-
 Nitrososphaera viennensis EN76 NO + oxidized phenazine methosulfate
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Nitrososphaera viennensis
? x * 39700, calculated from amino acid sequence Nitrososphaera viennensis

Synonyms

Synonyms Comment Organism
dissimilatory nitrite reductase
-
 Nitrososphaera viennensis
NirK
-
 Nitrososphaera viennensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 nitrite pH 6.5, 25°C Nitrososphaera viennensis
3.1
-
 nitrite at pH 8.0 and 25°C Nitrososphaera viennensis

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
 Nitrososphaera viennensis