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Literature summary for 1.7.2.3 extracted from

  • Silvestro, A.; Pommier, J.; Giordano, G.
    The inducible trimethylamine-N-oxide reductase of Escherichia coli K12: biochemical and immunological studies (1988), Biochim. Biophys. Acta, 954, 1-13.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 52% inhibition at 0.1 mM Escherichia coli
EGTA 38% inhibition at 10 mM Escherichia coli
KCN 20% inhibition at 1 mM Escherichia coli
NaN3 slight Escherichia coli
p-chloromercuribenzoate slight Escherichia coli
Urea 50% inhibition at 6 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 50% stimulation by 0.1 mM Fe3+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
a single inducible trimethylamine-N-oxide reductase which can exist as a dimer, MW: 230000, or a monomer, MW: 110000, gel filtration Escherichia coli
230000
-
a single inducible trimethylamine-N-oxide reductase which can exist as a dimer, MW: 230000 or a monomer, MW: 110000, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
inducible enzyme
-

Purification (Commentary)

Purification (Comment) Organism
using heat treatment, column chromatography on DEAE-cellulose, Sephadex G-200, DEAE-Sepharose CL-6B and hydroxyapatite Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
237
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, 45% loss of activity after 30 days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine N-oxide + electron donor
-
Escherichia coli adenosine + H2O + oxidized electron donor
-
?
chlorate + electron donor
-
Escherichia coli ?
-
?
hydroxylamine N-oxide + electron donor
-
Escherichia coli hydroxylamine + H2O + oxidized electron donor
-
?

Subunits

Subunits Comment Organism
dimer a single inducible trimethylamine-N-oxide reductase which can exist as a dimer, MW 230000 or a monomer, MW 110000 Escherichia coli
monomer a single inducible trimethylamine-N-oxide reductase which can exist as a dimer, MW 230000 or a monomer, MW 110000 Escherichia coli