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Literature summary for 1.7.2.3 extracted from
- Purification and properties of trimethylamine N-oxide reductase from aerobic photosynthetic bacterium Roseobacter denitrificans (1992), J. Biochem., 112, 470-475.
General Stability
| General Stability | Organism |
|---|---|
| activity is easily lost on dialysis | Roseobacter denitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
Trimethylamine N-oxide | - |
Roseobacter denitrificans |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | - |
Roseobacter denitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
2 * 90000, SDS-PAGE | Roseobacter denitrificans |
| 172000 | - |
gel filtration | Roseobacter denitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Roseobacter denitrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Roseobacter denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49.5 | - |
- |
Roseobacter denitrificans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C stable for several months | Roseobacter denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bromate + (ferrocytochrome c)-subunit | - |
Roseobacter denitrificans | ? + (ferricytochrome c)-subunit | - |
? | |
| hydroxylamine + (ferrocytochrome c)-subunit | - |
Roseobacter denitrificans | ? + (ferricytochrome c)-subunit | - |
? | |
| nicotinic acid N-oxide + (ferrocytochrome c)-subunit + H+ | - |
Roseobacter denitrificans | nicotinic acid + (ferricytochrome c)-subunit + H2O | - |
? | |
| picoline N-oxide + (ferrocytochrome c)-subunit + H+ | - |
Roseobacter denitrificans | picoline + (ferricytochrome c)-subunit + H2O | - |
? | |
| trimethylamine N-oxide + (ferrocytochrome c)-subunit + H+ | cytochrome c2, cytochrome c552 and methylviologen can serve as electron donors | Roseobacter denitrificans | trimethylamine + (ferricytochrome c)-subunit + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 90000, SDS-PAGE | Roseobacter denitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme c | - |
Roseobacter denitrificans | |
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