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Literature summary for 1.7.2.4 extracted from

  • Rasmussen, T.; Berks, B.C.; Butt, J.N.; Thomson, A.J.
    Multiple forms of the catalytic centre, Cuz, in the enzyme nitrous oxide reductase from Paracoccus pantotrophus (2002), Biochem. J., 364, 807-815.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
O2 inactivation is faster in the crude extract than solutions of the purified enzyme, acceleration takes place if the enzyme is turning over Paracoccus pantotrophus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu one dinuclear centre CuA and a copper cluster CuZ in which four copper ions are litigated by seven histidine imidazoles and a bridging inorganic sufide Paracoccus pantotrophus
Cu CuA can exist in two oxidation forms [Cu1.5+ - Cu1.5+] and [Cu1+ - Cu1+] Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrous oxide + reduced acceptor Paracoccus pantotrophus
-
nitrogen + H2O + acceptor
-
?

Organism

Organism UniProt Comment Textmining
Paracoccus pantotrophus
-
formerly Thiosphaera pantotropha
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
anaerobic form pre-reduced with ascorbate Paracoccus pantotrophus
2.6
-
anaerobic form pre-oxidized with ferricyanide Paracoccus pantotrophus
2.8
-
anaerobic form pre-reduced with dithionite Paracoccus pantotrophus
8.5
-
aerobic form pre-oxidized with ferricyanide Paracoccus pantotrophus
8.8
-
aerobic form pre-reduced with dithionite Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrous oxide + reduced acceptor
-
Paracoccus pantotrophus nitrogen + H2O + acceptor
-
?
nitrous oxide + reduced acceptor methyl viologen as electron donor, activity after incubation with ferricyanide, ascorbate or dithionite Paracoccus pantotrophus nitrogen + H2O + acceptor
-
?