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Literature summary for 1.7.2.4 extracted from
- Insights into the mechanism of N2O reduction by reductively activated N2O reductase from kinetics and spectroscopic studies of pH effects (2007), Inorg. Chem., 46, 613-615.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, overview | Achromobacter cycloclastes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a copper enzyme, conformational changes of the CuA center may affect electron transfer from the physiological electron donor and the electron-transfer rate from CuA to CuZ, overview | Achromobacter cycloclastes |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrogen + H2O + acceptor | Achromobacter cycloclastes | - |
nitrous oxide + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter cycloclastes | P94127 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 145.6 | - |
- |
Achromobacter cycloclastes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrogen + H2O + acceptor | - |
Achromobacter cycloclastes | nitrous oxide + reduced acceptor | - |
? | |
| nitrogen + H2O + acceptor | under some conditions, electron transfer may be rate limiting in N2O reduction. The transition state is stabilized by H-bonding interactions between the active site and an N2O-derived intermediate bound to the catalytic CuZ cluster | Achromobacter cycloclastes | nitrous oxide + reduced acceptor | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N2O reductase | - |
Achromobacter cycloclastes |
| N2OR | - |
Achromobacter cycloclastes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Achromobacter cycloclastes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 9.4 | pH profile with two maxima, high complexity of pH dependence, overview | Achromobacter cycloclastes |
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Release 2023.1 (January 2023)
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