Literature summary for 1.7.2.4 extracted from

Zhang, L.; Bill, E.; Kroneck, P.M.H.; Einsle, O.
Histidine-gated proton-coupled electron transfer to the CuA site of nitrous oxide reductase (2021), J. Am. Chem. Soc., 143, 830-838 .

Search for more literature for 1.7.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Stutzerimonas stutzeri

Protein Variants

Protein Variants	Comment	Organism
D576A	the mutant shows specific activity comparable to the wild type enzyme	Stutzerimonas stutzeri
D576A/S550A	the mutant shows decreased specific activity compared to the wild type enzyme	Stutzerimonas stutzeri
H583A	inactive	Stutzerimonas stutzeri
H583D	the oxidation rate of the CuA site in the mutant enzyme is much lower than that of wild type	Stutzerimonas stutzeri
H583E	the oxidation rate of the CuA site in the mutant enzyme is much lower than that of wild type	Stutzerimonas stutzeri
H583F	inactive	Stutzerimonas stutzeri
H583K	inactive	Stutzerimonas stutzeri
H583N	inactive	Stutzerimonas stutzeri
H583W	inactive	Stutzerimonas stutzeri
H583Y	the mutant has a redox-inert CuA site	Stutzerimonas stutzeri
H583Y/D576A	inactive although CuZ is still present	Stutzerimonas stutzeri

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	contains copper	Stutzerimonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
nitrous oxide + 2 ferrocytochrome c + 2 H+	Stutzerimonas stutzeri	-	nitrogen + H2O + 2 ferricytochrome c	-	?

Organism

Organism	UniProt	Comment	Textmining
Stutzerimonas stutzeri	P19573	i.e. Pseudomonas stutzeri	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrous oxide + 2 ferrocytochrome c + 2 H+	-	Stutzerimonas stutzeri	nitrogen + H2O + 2 ferricytochrome c	-	?
nitrous oxide + benzyl viologen + H+	-	Stutzerimonas stutzeri	nitrogen + oxidized benzyl viologen + H2O	-	?

Synonyms

Synonyms	Comment	Organism
N2OR	-	Stutzerimonas stutzeri
nitrous oxide reductase	-	Stutzerimonas stutzeri

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome c	-	Stutzerimonas stutzeri

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Release 2023.1 (January 2023)