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Literature summary for 1.7.2.5 extracted from

  • Blomberg, M.R.; Siegbahn, P.E.
    Improved free energy profile for reduction of NO in cytochrome c dependent nitric oxide reductase (cNOR) (2016), J. Comput. Chem., 37, 1810-1818 .
    View publication on PubMed
Search for more literature for 1.7.2.5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 nitric oxide + 2 ferrocytochrome c + 2 H+ Pseudomonas aeruginosa
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 nitrous oxide + 2 ferricytochrome c + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
nitrous oxide + 2 ferricytochrome c + H2O = 2 nitric oxide + 2 ferrocytochrome c + 2 H+ the reaction is nonelectrogenic, generation of a free energy profile for the entire catalytic cycle by hybrid density functional calculations. The energy profile shows that the reduction steps are endergonic and that the entire process is rate-limited by high proton uptake barriers during the reduction steps. Catalytic cycle and mechanism involving 2 different Fe atoms, modelling, detailed overview. Electrons and protons are taken from the same side of the membrane. The catalytic cycle can be divided into two parts. One part starts from the oxidized resting state with an oxo-bridge state 1, includes the two proton coupled reduction steps and results in the formation of a water molecule and the two-electron-reduced binuclear active site, states 3 and 4. The second part starts from the reduced state 4 and includes the main chemistry, that is, the reaction of two NO molecules with the reduced binuclear active site, formation and release of the nitrous oxide (N2O) molecule and ending up with the reformation of the oxidized state 1 Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 nitric oxide + 2 ferrocytochrome c + 2 H+
-
 Pseudomonas aeruginosa nitrous oxide + 2 ferricytochrome c + H2O
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 ?

Synonyms

Synonyms Comment Organism
cNOR
-
 Pseudomonas aeruginosa
cytochrome c dependent nitric oxide reductase
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 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
additional information quantum chemical calculations and combination of computational and experimental data for analysis of the reduction of nitric oxide (NO) to nitrous oxide and water in the membrane enzyme, cytochrome c dependent nitric oxide reductase (cNOR). Binuclear active site modelling and structure of the cis-hyponitrite intermediate, overview Pseudomonas aeruginosa