Literature summary for 1.7.2.5 extracted from

Sakurai, N.; Kataoka, K.; Sugaya, N.; Shimodaira, T.; Iwamoto, M.; Shoda, M.; Horiuchi, H.; Kiyono, M.; Ohta, Y.; Triwiyono, B.; Seo, D.; Sakurai, T.
Heterologous expression of Halomonas halodenitrificans nitric oxide reductase and its N-terminally truncated NorC subunit in Escherichia coli (2017), J. Inorg. Biochem., 169, 61-67 .

Search for more literature for 1.7.2.5

Cloned(Commentary)

Cloned (Comment)	Organism
gene norC, recombinant expression of wild-type and mutant NorC subunits in Escherichia coli strain BL21(DE3)	Halomonas halodenitrificans

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a truncated soluble domain of NorC, NorC* (DELTAMet1-Val37), by deletion of the 84 5'-terminal nucleotides of gene norC. The mutant NorC exhibits spectra typical of a low-spin heme c. In addition, NorC* functions as the acceptor of an electron from a cytochrome c isolated from the periplasm of Halomonas halodenitrificans and small reducing reagents. The redox potential of NorC* shifted ca. 40mV in the negative direction from that of wild-type NorC. Recombinant NOR exhibits the same spectroscopic properties and reactivity to NO and O2 as wil-type NOR, although its enzymatic activity toward NO is considerably decreased	Halomonas halodenitrificans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inner membrane	NorB is themembrane-spanning subunit	Halomonas halodenitrificans	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	subunit NorB contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center	Halomonas halodenitrificans

Organism

Organism	UniProt	Comment	Textmining
Halomonas halodenitrificans	O50651	small subunit encoded by gene norC; formerly Paracoccus halodenitrificans	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant NorC subunits from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and again ion exchange chromatography	Halomonas halodenitrificans

Subunits

Subunits	Comment	Organism
heterodimer	membrane-bound heterodimer complex of NorC, which contains a low-spin heme c center, and NorB, which contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center. NorB is themembrane-spanning subunit, it contains 12alpha-helices	Halomonas halodenitrificans

Synonyms

Synonyms	Comment	Organism
nitric oxide reductase	-	Halomonas halodenitrificans

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome c	-	Halomonas halodenitrificans
heme	subunit NorB contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center	Halomonas halodenitrificans

General Information

General Information	Comment	Organism
malfunction	recombinant truncated NOR exhibits the same spectroscopic properties and reactivity to NO and O2 as wil-type NOR, although its enzymatic activity toward NO is considerably decreased	Halomonas halodenitrificans
additional information	Halomonas halodenitrificans nitric oxide reductase (NOR) is the membrane-bound heterodimer complex of NorC, which contains a low-spin heme c center, and NorB, which contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center	Halomonas halodenitrificans
physiological function	NO reductase (NOR) protects denitrifying bacteria from NO, which is highly toxic, by converting it to N2O	Halomonas halodenitrificans

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Release 2023.1 (January 2023)