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Literature summary for 1.7.2.6 extracted from

  • Hooper, A.B.; Terry, K.R.
    Hydroxylamine oxidoreductase of Nitrosomonas. Production of nitric oxide from hydroxylamine (1979), Biochim. Biophys. Acta, 571, 12-20.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ce(NH4)2(NO3)6 0.1 mM, stimulates the rate of oxidation of NH2OH 2.6-fold and inhibits production of HNO2 by 40% Nitrosomonas europaea
Co(NO3)2 0.1 mM, 70% inhibition of the rate of nitrite synthesis Nitrosomonas europaea
H2O2 inhibits one step of oxidation of NH2OH to HNO Nitrosomonas europaea
Mn2+ complete inhibition of nitrite synthesis at 0.1 mM; complete inhibition of the oxidation of HNO to NO at 0.001 mM, inhibition of HNO2 synthesis is the same at pH 6,7,8 and 9 Nitrosomonas europaea

Metals/Ions

Metals/Ions Comment Organism Structure
Ce(NH4)2(NO3)6 0.1 mM, stimulates the rate of oxidation of NH2OH 2.6-fold and inhibits production of HNO2 by 40% Nitrosomonas europaea
Mn2+ 0.001 mM, stimulation of NH2OH utilization, stimulation at pH 6 and pH 9 is approximately 40% greater than at pH 7 and pH 8 Nitrosomonas europaea
additional information the presence of Mn2+ results in decreased formation of NO and increased formation of N2O Nitrosomonas europaea

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea
-
-
-
Nitrosomonas europaea Schmidt
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE cellulose column chromatography, gel filtration Nitrosomonas europaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + ferricytochrome c + H2O
-
Nitrosomonas europaea nitrite + ferrocytochrome c + H+
-
?
hydroxylamine + ferricytochrome c + H2O
-
Nitrosomonas europaea Schmidt nitrite + ferrocytochrome c + H+
-
?
additional information oxidation of hydroxylamine by the enzyme results in the production of a mixture of HNO2/HNO3 Nitrosomonas europaea ?
-
?
additional information oxidation of hydroxylamine by the enzyme results in the production of a mixture of HNO2/HNO3 Nitrosomonas europaea Schmidt ?
-
?

Synonyms

Synonyms Comment Organism
hydroxylamine oxidoreductase
-
Nitrosomonas europaea
hydroxylamine:oxygen oxidoreductase
-
Nitrosomonas europaea