Literature summary for 1.7.2.6 extracted from

Versantvoort, W.; Pol, A.; Jetten, M.; van Niftrik, L.; Reimann, J.; Kartal, B.; Op den Camp, H.
Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs (2020), Proc. Natl. Acad. Sci. USA, 117, 24459-24463 .

Search for more literature for 1.7.2.6

Inhibitors

Inhibitors	Comment	Organism	Structure
hydroxylamine	potent inhibitor	Methylacidiphilum fumariolicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0014	-	hydroxylamine	with bovine cytochrome c as electron acceptor, at pH 7.5 and 60°C	Methylacidiphilum fumariolicum

Organism

Organism	UniProt	Comment	Textmining
Methylacidiphilum fumariolicum	-	-	-
Methylacidiphilum fumariolicum SolV	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
SP Sepharose column chromatography and Superdex 200 gel filtration	Methylacidiphilum fumariolicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydroxylamine + 3 bovine ferricytochrome c	-	Methylacidiphilum fumariolicum	nitric oxide + 3 bovine ferrocytochrome c + 3 H+	-	?
hydroxylamine + 3 bovine ferricytochrome c	-	Methylacidiphilum fumariolicum SolV	nitric oxide + 3 bovine ferrocytochrome c + 3 H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 83860, calculated from amino acid sequence	Methylacidiphilum fumariolicum

Synonyms

Synonyms	Comment	Organism
hydroxylamine oxidoreductase	-	Methylacidiphilum fumariolicum
mHAO	-	Methylacidiphilum fumariolicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	the enzyme maintains about 80% of its maximum activity at temperatures above 60°C	Methylacidiphilum fumariolicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7.2	-	hydroxylamine	with bovine cytochrome c as electron acceptor, at pH 7.5 and 60°C	Methylacidiphilum fumariolicum

Cofactor

Cofactor	Comment	Organism	Structure
heme c	-	Methylacidiphilum fumariolicum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00052	-	hydroxylamine	at pH 7.5 and 60°C	Methylacidiphilum fumariolicum

General Information

General Information	Comment	Organism
physiological function	the enzyme allows methanotrophs to thrive under high ammonia concentrations in natural and engineered ecosystems, such as those observed in rice paddy fields, landfills, or volcanic mud pots, by preventing the accumulation of inhibitory hydroxylamine	Methylacidiphilum fumariolicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5100	-	hydroxylamine	with bovine cytochrome c as electron acceptor, at pH 7.5 and 60°C	Methylacidiphilum fumariolicum

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Release 2023.1 (January 2023)