Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | strong competitive inhibitor | Candidatus Kuenenia stuttgartiensis | |
nitric oxide | strong competitive inhibitor | Candidatus Kuenenia stuttgartiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
hydrazine | at pH 7.0 and 37°C | Candidatus Kuenenia stuttgartiensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
3 * 67000, SDS-PAGE | Candidatus Kuenenia stuttgartiensis |
200000 | - |
gel filtration | Candidatus Kuenenia stuttgartiensis |
201800 | - |
MALDI-TOF mass spectrometry | Candidatus Kuenenia stuttgartiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydrazine + ferricytochrome c | Candidatus Kuenenia stuttgartiensis | highly specific for hydrazine oxidation activity | N2 + ferrocytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candidatus Kuenenia stuttgartiensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography and hydroxyapatite column chromatography | Candidatus Kuenenia stuttgartiensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | - |
crude extract, at pH 7.0 and 37°C | Candidatus Kuenenia stuttgartiensis |
8.4 | - |
after 15fold purification, at pH 7.0 and 37°C | Candidatus Kuenenia stuttgartiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydrazine + ferricytochrome c | highly specific for hydrazine oxidation activity | Candidatus Kuenenia stuttgartiensis | N2 + ferrocytochrome c | - |
? | |
additional information | hydroxylamine does not serve as a substrate | Candidatus Kuenenia stuttgartiensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 67000, SDS-PAGE | Candidatus Kuenenia stuttgartiensis |
homotrimer | 3 * 62272, calculated from amino acid sequence | Candidatus Kuenenia stuttgartiensis |
Synonyms | Comment | Organism |
---|---|---|
HDH | - |
Candidatus Kuenenia stuttgartiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | hydrazine | at pH 7.0 and 37°C | Candidatus Kuenenia stuttgartiensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the enzyme possessesg eight bound heme molecules | Candidatus Kuenenia stuttgartiensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
nitric oxide | at pH 7.0 and 37°C | Candidatus Kuenenia stuttgartiensis | |
0.0079 | - |
hydroxylamine | at pH 7.0 and 37°C | Candidatus Kuenenia stuttgartiensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3500 | - |
hydrazine | at pH 7.0 and 37°C | Candidatus Kuenenia stuttgartiensis |