Protein Variants | Comment | Organism |
---|---|---|
additional information | uricase covalently linked to monomethoxypoly(ethylene glycol) N-leucine-OSu, branched monomethoxypoly(ethylene glycol) N-leucine-OSu or poly(N-acryloylmorpholine)-OSu last longer in blood | Metabacillus fastidiosus |
General Stability | Organism |
---|---|
conjugated uricases are more stable to trypsin digestion | Metabacillus fastidiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
uric acid | poly(N-acryloylmorpholine)-OSu-uricase | Metabacillus fastidiosus | |
0.05 | - |
uric acid | native uricase, monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase and branched monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase | Metabacillus fastidiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | Metabacillus fastidiosus | - |
5-hydroxyisourate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metabacillus fastidiosus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Metabacillus fastidiosus | 5-hydroxyisourate + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
uricase covalently linked to monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase and branched monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase 50% loss of activity | Metabacillus fastidiosus |
60 | 61 | native uricase and poly(N-acryloylmorpholine)-OSu-uricase 50% loss of activity | Metabacillus fastidiosus |