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Literature summary for 1.7.3.3 extracted from

  • Montalbini, P.; Aguilar, M.; Pineda, M.
    Isolation and characterization of uricase from bean leaves and its comparison with uredospore enzymes (1999), Plant Sci., 147, 139-147.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
oxonate 0.025 mM 90% inhibition Phaseolus vulgaris
oxonate 0.025 mM 90% inhibition Uromyces phaseoli
oxonate 0.025 mM 90% inhibition Uromyces viciae-fabae
oxonate
-
Vicia faba
xanthine 0.05 mM 60-75% inhibition Phaseolus vulgaris
xanthine 0.05 mM 60-75% inhibition Uromyces phaseoli
xanthine 0.05 mM 60-75% inhibition Uromyces viciae-fabae
xanthine
-
Vicia faba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
uric acid
-
Phaseolus vulgaris
0.021
-
uric acid
-
Vicia faba
0.032
-
uric acid
-
Uromyces phaseoli
0.034
-
uric acid
-
Uromyces viciae-fabae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000 122000
-
Vicia faba
124000
-
PAGE Uromyces viciae-fabae
124000
-
PAGE Uromyces phaseoli
128000
-
PAGE Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Phaseolus vulgaris
-
5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O Vicia faba
-
5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O Uromyces viciae-fabae
-
5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O Uromyces phaseoli
-
5-hydroxyisourate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-
Uromyces phaseoli
-
-
-
Uromyces viciae-fabae
-
-
-
Vicia faba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Phaseolus vulgaris
-
Uromyces viciae-fabae
-
Uromyces phaseoli

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Phaseolus vulgaris
-
leaf
-
Vicia faba
-
uredospore
-
Uromyces viciae-fabae
-
uredospore
-
Uromyces phaseoli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.87
-
-
Phaseolus vulgaris
2.04
-
-
Uromyces viciae-fabae
3.11
-
-
Uromyces phaseoli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Phaseolus vulgaris 5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O
-
Vicia faba 5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O
-
Uromyces viciae-fabae 5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O
-
Uromyces phaseoli 5-hydroxyisourate + H2O2
-
?

pH Range

pH Minimum pH Maximum Comment Organism
8 9
-
Uromyces phaseoli
8.5 9.1
-
Uromyces viciae-fabae
8.6 9.5
-
Vicia faba
9 9.7
-
Phaseolus vulgaris