Activating Compound | Comment | Organism | Structure |
---|---|---|---|
uric acid | uric acid (0.3%) is an inducer for uricase production, concentrations higher than 0.3% do not enhance the enzyme productivity | Geobacillus thermocatenulatus |
Application | Comment | Organism |
---|---|---|
medicine | determination of the urate concentration in blood and urine is required for the diagnosis of gout as urate accumulation is a causative factor of gout in humans | Geobacillus thermocatenulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
uric acid | 0.1 M sodium borate, pH 8.5 containing 2 mM uric acid, 30 mM 4-aminoantipyrine, 1.5% phenol and peroxidase (15 U/ml), 37°C, 20 min | Geobacillus thermocatenulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | Geobacillus thermocatenulatus | ureide pathway | 5-hydroxyisourate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermocatenulatus | DQ887577 | partial sequence | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activity is 0.06 U/ml at pH 4.0 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.09 U/ml at pH 4.5 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.11 U/ml at pH 5.0 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.21 U/ml with potassium nitrate as nitrogen source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.22 U/ml at pH 10.0 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.31 U/ml with ammonium sulfate as nitrogen source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.32 U/ml with sodium glutamate as nitrogen source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.37 U/ml at pH 5.5 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.42 U/ml at pH 9.5 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.45 U/ml with citric acid as carbon source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.46 U/ml with glucose as carbon source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.46 U/ml with peptone as nitrogen source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.47 U/ml at pH 6.0 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.47 U/ml with lactose as carbon source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.49 U/ml with starch as carbon source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.57 U/ml at pH 9.0 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.57 U/ml with soybean flour as nitrogen source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.63 U/ml at pH 8.5 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.65 U/ml with beef extract as nitrogen source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.67 U/ml with sucrose as carbon source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.69 U/ml with yeast extract as nitrogen source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.72 U/ml at pH 8.0 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.78 U/ml with maize milk as nitrogen source | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 0.98 U/ml at pH 6.5 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 1.00 U/ml at pH 7.5 | Geobacillus thermocatenulatus |
additional information | - |
enzyme activity is 1.25 U/ml at pH 7.0 | Geobacillus thermocatenulatus |
additional information | - |
when the strain is cultured at 30°C at pH 7.0 for 30-36 h with of 0.6% corn steep liquor as nitrogen source, the uricase activity peaks at 1.25 U/ml | Geobacillus thermocatenulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | ureide pathway | Geobacillus thermocatenulatus | 5-hydroxyisourate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Urate oxidase | - |
Geobacillus thermocatenulatus |
uricase | - |
Geobacillus thermocatenulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
enzyme is thermostable, after heat treatment at 75°C for 45 min, the uricase retains about 100% of its initial activity | Geobacillus thermocatenulatus |
80 | - |
70% of initial activity remains after 45 min at 80°C | Geobacillus thermocatenulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
optimal pH for uricase production in culture flasks | Geobacillus thermocatenulatus |
8.5 | - |
assay at | Geobacillus thermocatenulatus |