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Literature summary for 1.7.3.3 extracted from

  • Gabison, L.; Prange, T.; Colloch, N.; El Hajji, M.; Castro, B.; Chiadmi, M.
    Structural analysis of urate oxidase in complex with its natural substrate inhibited by cyanide: mechanistic implications (2008), BMC Struct. Biol., 8, 32.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with substrate urate and inhibitor cyanide, X-ray diffraction structure determination and analysis Aspergillus flavus

Inhibitors

Inhibitors Comment Organism Structure
cyanide
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Aspergillus flavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Aspergillus flavus urate oxidase catalyzes the hydroxylation of uric acid into the metastable product 5-hydroxyisourate in the presence of molecular oxygen as part of the purine degradation pathway. 5-Hydroxyisourate decays slowly to allantoin, a process independent of oxygen and associated with the release of CO 5-hydroxyisourate + H2O2
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Organism

Organism UniProt Comment Textmining
Aspergillus flavus Q00511
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Reaction

Reaction Comment Organism Reaction ID
urate + O2 + H2O = 5-hydroxyisourate + H2O2 catalytic mechanism, overview Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O urate oxidase catalyzes the hydroxylation of uric acid into the metastable product 5-hydroxyisourate in the presence of molecular oxygen as part of the purine degradation pathway. 5-Hydroxyisourate decays slowly to allantoin, a process independent of oxygen and associated with the release of CO Aspergillus flavus 5-hydroxyisourate + H2O2
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urate + O2 + H2O substrate binding involves residues Arg176 and Glu228, that hold the substrate, Phe159 closing one end of the cavity below, and the two residues Asn254 and Thr57, forming another tweezers above the mean plane of the ligand that construct a location where efficient electron transfer can take place at a low energy level via the catalytic triad Thr57, Lys10, and His256 Aspergillus flavus 5-hydroxyisourate + H2O2
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Synonyms

Synonyms Comment Organism
Uox
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Aspergillus flavus
uricase
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Aspergillus flavus