Literature summary for 1.7.3.3 extracted from

Feng, T.; Yang, X.; Wang, D.; Hu, X.; Liao, J.; Pu, J.; Zhao, X.; Zhan, C.G.; Liao, F.
A practical system for high-throughput screening of mutants of Bacillus fastidiosus uricase (2017), Appl. Biochem. Biotechnol., 181, 667-681 .

Search for more literature for 1.7.3.3

Application

Application	Comment	Organism
analysis	development of a high-throughput screening system of Bacillus fastidiosus uricase mutants, using 96-well plates and monitoring of uric acid by absorbance at 298 nm	Metabacillus fastidiosus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Metabacillus fastidiosus

Protein Variants

Protein Variants	Comment	Organism
L171I/Y182F/Y187F/A193S	mutant displays higher activity and lower thermostability	Metabacillus fastidiosus
V144A	mutant displays higher activity and consistent thermostability	Metabacillus fastidiosus

General Stability

General Stability	Organism
half-life of wild-type at pH 7.4, 37°C is 22 days, half-life of mutant L1711I/Y182F/Y187F/A193S is 0.3 days	Metabacillus fastidiosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.27	-	Urate	mutant L1711I/Y182F/Y187F/A193S, pH 9.2, 27°C	Metabacillus fastidiosus
0.31	-	Urate	wild-type, pH 9.2, 27°C	Metabacillus fastidiosus

Organism

Organism	UniProt	Comment	Textmining
Metabacillus fastidiosus	C5HDG5	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.8	-	wild-type, pH 9.2, 27°C	Metabacillus fastidiosus
8.3	-	mutant L1711I/Y182F/Y187F/A193S, pH 9.2, 27°C	Metabacillus fastidiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
urate + O2 + H2O	-	Metabacillus fastidiosus	5-hydroxyisourate + H2O2	-	?

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Release 2023.1 (January 2023)