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Literature summary for 1.7.3.3 extracted from

  • Ravichandran, R.; Hemaasri, S.; Cameotra, S.S.; Jayaprakash, N.S.
    Purification and characterization of an extracellular uricase from a new isolate of Sphingobacterium thalpophilum (VITPCB5) (2015), Protein Expr. Purif., 114, 136-142 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 20% residual activity Sphingobacterium thalpophilum
EDTA 1 mM, complete loss of activity Sphingobacterium thalpophilum
Fe2+ 1 mM, 70% residual activity Sphingobacterium thalpophilum
Ni2+ 1 mM, 60% residual activity Sphingobacterium thalpophilum
Zn2+ 1 mM, 60% residual activity Sphingobacterium thalpophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
Urate pH 8.5, 25°C Sphingobacterium thalpophilum

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1 mM, 140% of initial activity Sphingobacterium thalpophilum

Organism

Organism UniProt Comment Textmining
Sphingobacterium thalpophilum JX083378
-
-
Sphingobacterium thalpophilum VITPCB5 JX083378
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Sphingobacterium thalpophilum 5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O
-
Sphingobacterium thalpophilum VITPCB5 5-hydroxyisourate + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Sphingobacterium thalpophilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45
-
Sphingobacterium thalpophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sphingobacterium thalpophilum

pH Range

pH Minimum pH Maximum Comment Organism
6
-
more than 80% of maximum activity Sphingobacterium thalpophilum
9
-
more than 80% of maximum activity Sphingobacterium thalpophilum

Expression

Organism Comment Expression
Sphingobacterium thalpophilum maximum expression upon growth on lactose as carbon source and urea as nitrogen source up